FastQCFastQC Report
Wed 25 May 2016
SRR1780044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73318
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4529561.778826481900765No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG59288.08532693199487No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT7300.9956627294798003No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC5870.800621948225538No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG5840.7965301835838403No Hit
TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG1980.27005646635205544No Hit
CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG1210.16503450721514498No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1060.14457568400665594No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCATGTGTAGATCTCGGTG760.10365803758967784No Hit
TATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGT740.10093019449521264No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAT150.002216581370.012
CGATACA207.879279E-570.025
AGAAGGT150.002216581370.025
GATACAC207.879279E-570.026
AGAACAT150.002216581370.032
TGTTAAA150.002216581370.021
ATAGTCA150.002216581370.09
TTGATAG150.002216581370.06
ATATTGG150.002216581370.015
AGTCATA150.002216581370.011
TTTGATA150.002216581370.05
AGTGTAG252.376248E-455.99999635
CTATACA452.6571797E-854.4444431
AAAAAGG5300.052.83018570
GAAACCC200.00692622952.551
AATTCCT200.00692622952.552
CCTGCCC200.00692622952.519
TAGTCAT200.00692622952.510
TGATAGT200.00692622952.57
CATTAAC200.00692622952.536
Produced by FastQC (version 0.11.5)