FastQCFastQC Report
Wed 25 May 2016
SRR1780044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73318
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3061641.757822090073375No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG994613.565563708775471No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG36024.912845413131837No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC22943.1288360293515916No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT13831.8863034998226902No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT8181.1156878256362694Illumina PCR Primer Index 6 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC6970.9506533184211244No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT4050.5523882266292043No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC3430.46782509070078293No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCCAAAATCTCGTATGCCGT2430.33143293597752255No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT2220.30279058348563787No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1830.24959764314356636No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1610.2195913691044491No Hit
TATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCT1600.21822744755721651Illumina PCR Primer Index 6 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1450.1977686243487275No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC1450.1977686243487275No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG1430.19504078125426225No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC1400.19094901661256444No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC1310.17867372268747103No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGACGT1300.17730980114023842No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT1090.14866744864835374No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT950.1295725469870973No Hit
CACTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC950.1295725469870973No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAACTCTCGTATGCCGT860.11729725306200388No Hit
CAAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT850.11593333151477127No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCAGT790.10774980223137566No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAATC207.879279E-570.06
CTTTTCC150.002216581370.016
GAGGCAG150.002216581370.041
ACAAAGG150.002216581370.066
ACTCGTA252.8145296E-670.038
AAACTCG252.8145296E-670.036
CCAACAT150.002216581370.032
CTTTACA252.8145296E-670.01
TCTAGAA150.002216581370.038
ATGACTA150.002216581370.026
ACCACGA252.8145296E-670.019
TATCGTA150.002216581370.038
CATCTCG207.879279E-570.036
ACTCTTT150.002216581370.047
ACCAAAA150.002216581370.031
AGAATGC150.002216581370.041
AACTCGT207.879279E-570.037
TCAAAAG150.002216581370.033
AAGATCT150.002216581370.037
GAACCAA207.879279E-570.029
Produced by FastQC (version 0.11.5)