FastQCFastQC Report
Wed 25 May 2016
SRR1780043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79579
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4764259.86755299765013No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG66468.351449503009588No Hit
TCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGT7160.8997348546727152No Hit
ATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGGTC7020.8821422737154274No Hit
CTCTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG5300.6660048505258925No Hit
TCTTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGG2290.28776435994420635No Hit
CTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTGG1510.18974855175360333No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1340.16838613201975394No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCATGTGTAGATCTCGGTG820.10304225989268526No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGC207.88341E-570.000011
TTTTGCT150.002217278870.068
GTAGAAC150.002217278870.038
GGCGCGT150.002217278870.05
AAATATA150.002217278870.017
GGTTTTG150.002217278870.066
CAGGGCG150.002217278870.04
ACGGTGG150.002217278870.045
TTTGCTG150.002217278870.069
CGCGTCT150.002217278870.07
TCAATAA308.308909E-658.33333260
TAGAACT252.3774867E-455.99999639
GCGCGTC252.3774867E-455.9999966
GTCTGGC252.3774867E-455.99999610
TTGTGCG252.3774867E-455.9999962
AGGGCGG200.006928391852.5000045
GTTTTGC200.006928391852.50000467
AGAACTC200.006928391852.50000440
AACTCGG200.006928391852.50000442
CTGGCGC200.006928391852.50000412
Produced by FastQC (version 0.11.5)