FastQCFastQC Report
Wed 25 May 2016
SRR1780043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79579
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT3066538.534035361087724No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1081213.586498950728206No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG33904.259917817514671No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC25683.226981992736777No Hit
CATATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT10061.2641526030736752No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCGGTCATCTCGTATGCCGT5470.6873672702597419No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT4970.6245366239837142Illumina PCR Primer Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC4950.6220233981326732No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCGT3910.49133565387853584No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT3590.4511240402618782No Hit
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC2820.35436484499679566No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT2670.33551565111398735No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT1930.2425262946254665No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTAAGCCGT1850.23247339122130212No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG1770.2224204878171377No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG1600.20105806808328833No Hit
CTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT1400.17592580957287726No Hit
CTTTACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC1270.1595898415411101No Hit
TTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC1260.15833322861558954No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC1210.1520501639879868No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCAT1140.1432538735093429No Hit
TATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCT1090.13697080888174015No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG1000.1256612925520552No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATATCGTATGCCGT960.12063484084997297No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCG920.11560838914789076No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAC150.002211830670.0433961
AATTCTG150.002217357769.99937450
ATAAGCA207.883761E-569.99937452
CACGTAA150.002217357769.99937439
GAAAAAT150.002217357769.99937424
CCGTCTA207.883761E-569.99937447
TTCCCAG150.002217357769.99937424
TATCCGA150.002217357769.99937411
ACGAGAA150.002217357769.99937422
CGAATTC150.002217357769.99937448
TCATGAA150.002217357769.99937456
AAAGCCG150.002217357769.99937443
ATATCCG150.002217357769.99937410
CCGAATT150.002217357769.99937447
CTACATG150.002217357769.99937454
CTGATTG252.8168924E-669.9993754
CTTCTTG252.8168924E-669.9993754
CGTCTAC252.8168924E-669.9993748
AGGGGGG33000.064.2721570
ATATACA1850.062.431872
Produced by FastQC (version 0.11.5)