FastQCFastQC Report
Wed 25 May 2016
SRR1780042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333884
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3760411.262594194390866No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG86492.59042062512729No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT10190.30519581651112365No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG10110.30279977477207654No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC9050.27105222172970256No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACGG150.002223819769.99760419
ACGGTTC207.921734E-569.99760423
AGTCGCT252.3889761E-455.9980818
ACGTTTG200.006948678352.49820314
TCCTCGT200.006948678352.49820310
TCCTCGA200.006948678352.49820340
GCTTCGT200.006948678352.4982031
TCGTCGG200.006948678352.49820313
ATGAACG200.006948678352.49820318
CGATCGA200.006948678352.4982031
ACGACGT200.006948678352.49820356
GCCGTAT15400.047.04384654
TCGCCGT15650.046.06871452
CGTATCA15750.045.99842556
CCGTATC15850.045.92902855
TCGGTGG16050.045.57476445
TGGTCGC15900.045.5644849
TCTCGGT16150.045.50927743
ATCATTA16100.045.43322859
CGGTGGT16000.045.27969746
Produced by FastQC (version 0.11.5)