FastQCFastQC Report
Wed 25 May 2016
SRR1780042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333884
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT147214.409016305064034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140864.218830492027171No Hit
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT84922.543398305998491No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG27550.8251368738843431No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC22240.6660996034550922No Hit
CACTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC9170.27464628433827315No Hit
CATAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT7320.21923781912280912No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC5410.16203232260305975No Hit
CAAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT4500.13477734782139905No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT4420.13238130608235193No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT3690.11051742521354722TruSeq Adapter, Index 11 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTCT150.002224329669.9935615
TGGTCGC150.002224329669.9935633
AGGGGGG31550.062.89436770
CATATAC11600.059.4341851
ATATACA11850.058.4756362
TAACTCG252.3896576E-455.9948536
AGCATCG200.00693792452.51876428
CGACTTT200.00693792452.51876426
AAGGGGG37850.052.51828469
TCGTGTC200.006946150752.50303363
CGGGTAC200.00695026752.4951739
CCGGAAT200.00695026752.4951756
TCGATCG200.00695026752.4951750
TCGTGAC200.00695026752.4951712
TTCGACA200.00695026752.495174
CCGTCTT36450.052.4231647
TATGCCG37700.052.35592343
CGTCTTC36700.052.3521348
GCCGTCT36800.052.3049746
GTATGCC37850.052.05597742
Produced by FastQC (version 0.11.5)