FastQCFastQC Report
Wed 25 May 2016
SRR1780041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431364
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG416079.64545024619579No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG81971.9002512958893187No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG12250.2839829007520331No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC10590.24550031991543106No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT7450.17270796821246095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCG150.00222314270.0064916
CGTCCAC200.00694655952.5048731
AACGTTT200.006949744752.49878321
GCGAAGT451.6822505E-646.6709932
GCCGTAT13500.046.1470854
TCGCCGT13600.045.80776252
TGGTCGC13950.045.66202549
TCGGTGG14050.045.5861345
CTCGGTG13950.045.41113344
CGGTGGT14050.045.0879246
GTCGCCG14000.044.9989651
ATCTCGG14100.044.92803242
TCTCGGT14350.044.38921443
GATCTCG14300.044.29967541
CGCCGTA14100.044.1833753
AGATCTC14350.044.14531740
GTAGATC14450.043.83981738
GATCATA404.590638E-543.748991
CGTATCA14400.043.74898556
GTATCAT14650.043.7191257