FastQCFastQC Report
Wed 25 May 2016
SRR1780041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431364
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT162413.7650337070316486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140493.2568781817676022No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT91792.127901262043193No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC31590.7323281497760592No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG28660.6644040763716954No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC11520.26705983809497313No Hit
CATAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT7940.18406728424254226No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC5080.11776597027104718No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT4700.10895670477833105TruSeq Adapter, Index 9 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT4680.10849305922608285No Hit
CAAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT4670.10826123644995873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG35850.060.624970
ATATCGT509.822543E-1055.99100537
CGAATGC452.701745E-854.43569641
ATATACA13150.053.4895062
CATATAC13000.053.2991261
AAGGGGG40900.052.88271769
CGTCTTC43000.050.7070348
GCCGTCT43100.050.50232746
CCGTCTT42750.050.42464447
TATGCCG43400.050.1532343
CGTATGC43800.049.7751141
TCTCGTA43650.049.70564338
ATCTCGT43600.049.6823937
CTCGTAT43750.049.6720239
CTGCTTG42700.049.6699154
TCGTATG44200.049.64134240
GTATGCC43800.049.5354242
ATGCCGT44350.049.39454744
TCTTCTG43650.049.39068650
GTCTTCT43650.049.39068649
Produced by FastQC (version 0.11.5)