FastQCFastQC Report
Wed 25 May 2016
SRR1780040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448880
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG437399.744029584744252No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG90102.0072179647121726No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG12360.27535198716806275No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC11460.25530208518980574No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT9260.20629121368739975No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACAG150.002223133870.0070228
CTCGACG200.006949597552.4994163
TCGACGC200.006949597552.4994164
GCCGTAT15350.045.14607654
GATCTCG15450.045.08040641
TCGCCGT15400.044.77223252
CGGTGGT15700.044.5854946
ATCTCGG15650.044.50429542
GTCGCCG15600.044.42258551
TGGTCGC15600.044.19822749
CTCGGTG15850.044.16354844
AGATCTC15900.044.02466640
TCGGTGG15950.043.88665845
GCTTCTA404.5906123E-543.749511
GTAGATC16050.043.6132238
CGTATCA16000.043.5307656
TCTCGGT16150.043.3431743
CGCCGTA15850.043.28027753
TAGATCT16250.043.07644339
GTATCAT16200.042.99334757
Produced by FastQC (version 0.11.5)