FastQCFastQC Report
Wed 25 May 2016
SRR1780040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448880
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT161793.6043040456246658No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158393.5285599714845834No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT93572.0845214756727857No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC31400.6995188023525218No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG29620.6598645517733024No Hit
CACTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC11420.25441097843521654No Hit
CATAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT10110.22522723222242028No Hit
CAAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT6220.13856710033862055No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT5620.12520049901978259No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT4520.10069506326857959TruSeq Adapter, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA252.8338E-670.0181627
CGCGAAT252.8338E-670.0181628
ACGTATG150.002224678669.9947740
CCGACTT501.4551915E-1162.99528547
AGGGGGG37400.060.54360270
AAACTCG352.9733928E-759.9955136
GACGCGA308.35894E-658.34846526
CATATAC13950.053.191911
GCTCTAC200.00694828552.501921
GCTAACG200.00694828552.501922
GAGGACG200.00695134752.4960714
CGTCTAC407.477902E-752.4960748
TAACGTG200.00695134752.496074
AGACGAT200.00695134752.496077
GCCGACA200.00695134752.4960746
TACCTTG200.00695134752.496076
ACTCGAT200.00695134752.4960759
GATTGTC200.00695134752.4960763
CGGTATG200.00695134752.4960767
GCCGTAT200.00695134752.4960746
Produced by FastQC (version 0.11.5)