FastQCFastQC Report
Wed 25 May 2016
SRR1780039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609422
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450147.386343125125118No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG82801.3586644394196468No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG11450.18788294482312748No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT10100.1657308072238941No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC9890.16228491915290227No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTG150.002223667270.0058337
AGCGCGA207.9255864E-570.0000846
CCGTAAC200.00694593552.5086856
GAACACG200.00694593552.5086831
CAGCGTA200.006950445552.500061
GCAAGCG200.006950445552.500063
TCGGGAA200.00695495852.49144412
ACGAGAC200.00695495852.49144448
GCCGTAT14500.044.1760954
GGTACAT404.5922214E-543.750051
CGGTGGT15000.043.3929346
TCGCCGT14800.043.0370652
ATCTCGG15250.042.91808342
TCGGTGG15300.042.7708145
GATCTCG15400.042.7273241
GTCGCCG15000.042.6965551
CTCGGTG15450.042.12905544
CGCCGTA15250.042.00349853
AGATCTC15700.041.91431840
TGGTCGC15550.041.8615749