FastQCFastQC Report
Wed 25 May 2016
SRR1780039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609422
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT169042.773775807240303Illumina PCR Primer Index 6 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142542.338937550662759No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT100661.6517290153620972Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG28590.46913304737932005No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC26130.4287669299762726No Hit
CACTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC9720.15949539071448027No Hit
CATAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT8570.1406250512780963Illumina PCR Primer Index 6 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA1000.059.50069841
AGGGGGG39000.058.87731670
ACGTATG553.6379788E-1157.27809540
CGTCTTA509.822543E-1055.99146748
ATATACA16300.052.6166152
AAACGAC200.00695241152.4963076
TATGCCG45200.051.7262743
GCCGTCT44000.051.4665146
CCGTCTT43900.051.3445747
CGTATGC45900.051.24242841
CGTCTTC44100.051.1826848
TGCCGTC45250.051.1276745
TCGTATG46550.051.05742340
ATGCCGT45700.051.00716444
GTATGCC46000.050.90277542
CTCGTAT46750.050.7641239
CTGCTTG43500.050.60148254
CATATAC16550.050.341181
CTTCTGC44150.050.2528251
AAGGGGG45450.050.20961469