FastQCFastQC Report
Wed 25 May 2016
SRR1780038_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780038_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662732
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG424966.412245070405534No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG92501.395737643572364No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC11970.180615995606067No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG11480.17322235835903502No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT10350.1561717255240429No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGC252.3907161E-455.99800512
GGACGTT200.00694342952.5139830
CGTTCGA200.006945501552.51001733
GGTCCGA200.00695172352.498131
GCCGGTA200.00695172352.4981348
CTGGTAC200.00695172352.4981355
ATACGCG200.00695172352.498134
ACCCGAG200.00695379852.494177
GCCGTAT17300.045.7209254
TCGGTGG17450.045.327945
CTCGGTG17350.045.1857144
TCGCCGT17350.045.182352
TGGTCGC17500.044.99839849
GTCGCCG17600.044.73935351
CGGTGGT17700.044.4899446
GATCTCG17650.044.4176841
CGCCGTA17850.044.31215353
ATCTCGG17750.044.16744242
TCTCGGT17950.044.0686143
AGATCTC17950.043.87030840