FastQCFastQC Report
Wed 25 May 2016
SRR1780038_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780038_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662732
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160872.4273763753674187No Hit
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT160322.4190773947840154No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT91191.375970980728258No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC27890.42083376085657553No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG27820.4197775269641424No Hit
CACTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC9660.1457602771557734No Hit
CATAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT9230.13927198324511267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG37200.057.8660870
CCGACTT252.3905377E-455.9988547
GCCGTCA1900.055.26202846
TATGCCG42550.050.17523643
CCGTCTT40600.050.17138747
CGTATGC43150.050.12643441
ATCGTAT352.0858326E-550.0027539
TCGTATG43850.049.9685440
CGTCTTC40800.049.83590348
GCCGTCT41350.049.68459346
TGCCGTC43050.049.42988245
ATGCCGT43550.049.42493444
CGAATGC850.049.41075541
CTCGTAT44150.049.31188639
CTTGAAA43750.049.03529457
GTATGCC43900.048.87143342
TCTCGTA44500.048.84538338
ATCTCGT44400.048.71889537
AAGGGGG44150.048.67397369
TTGAAAA44400.048.55756458
Produced by FastQC (version 0.11.5)