FastQCFastQC Report
Wed 25 May 2016
SRR1780037_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780037_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307717
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4538814.749916319215384No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG87422.8409220160082156No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC12290.39939294871586556No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT10060.3269237643679095No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG9990.3246489469220095No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG150.002222410470.0074831
TTCGCGA150.002222410470.0074832
TCGCGAT150.002222410470.0074833
GCGATAC150.002223843569.996135
TATACGC207.921591E-569.99613
TGGACGC252.3889348E-455.9968849
GTACTAG252.3889348E-455.996881
CATTCGC200.006939825652.5141430
CCGGCAC200.006944288552.5056124
TTGACGC200.006944288552.5056132
TTTGACG200.006944288552.5056131
CGAGTCA200.006948753752.49707815
TCCGATA200.006948753752.49707821
GGCCGAT200.006948753752.4970781
GCCGGCA200.006948753752.49707823
GTGGACG200.006948753752.49707848
GTGCAAC451.6805025E-646.6716524
AAACTGG305.87427E-446.66406616
TGCGGAG305.87427E-446.6640665
TCGGTGG15150.046.43305645
Produced by FastQC (version 0.11.5)