FastQCFastQC Report
Wed 25 May 2016
SRR1780037_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780037_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307717
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT156635.090066522161597No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148554.827487594120572No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT101073.284511417958709No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG29160.9476239531777574No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC25780.8377827679328733No Hit
CACTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC10690.3473971213810092No Hit
CATAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT8690.2824023372124387No Hit
CAAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT5570.18101047390946876No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT4910.1595621951338405TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT4730.15371266455866917No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC4580.14883805574602638No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT4560.14818810790434067No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT4540.14753816006265497No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGAG150.002224068669.9943117
CCTGGTC150.002224068669.9943116
AACTCGA150.002224068669.9943137
CGAATGC550.063.63119541
TCGAATG501.4551915E-1162.99488440
AGGGGGG37500.062.9948870
CATATAC14600.059.2075231
AAAACGG308.361547E-658.33807467
ATATACA15000.058.328592
ACGTATG308.369607E-658.3285940
CGACTTC252.3892355E-455.99545348
CACGTAT252.3892355E-455.99545339
AAGGGGG43900.053.5719669
CGCGGCT200.006940527452.512831
GCGTGAC200.006944990752.5042763
GACGTCG200.00694945652.4957357
AGGACAC200.00694945652.4957356
TTCCGTG200.00694945652.49573570
TCGCTAT200.00694945652.49573549
CGTCTTA608.367351E-1152.4957348
Produced by FastQC (version 0.11.5)