FastQCFastQC Report
Wed 25 May 2016
SRR1780036_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780036_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125956
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4507235.78392454507923No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG122099.693067420369019No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT13441.067039283559338No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG13141.04322144240846No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC12641.00352504049033No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG3280.26040839658293374No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG2620.20800914605100196No Hit
TATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGT1360.10797421321731399No Hit
CTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT1300.10321064498713838No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA150.00222029570.000011
AGTCATC150.00222029570.000018
TTGTGCG150.00222029570.000012
GAACTCA150.00222029570.0000116
GGATCGA150.00222029570.0000121
AGGACAG150.00222029570.0000148
TGTCCCT150.00222029570.0000110
TGTGCGC207.901282E-570.03
GTTCGCA207.901282E-570.09
TCTTTAT353.6779966E-970.01
CTTTATA401.0570147E-861.252
TGTGGGC308.337027E-658.3333363
GGCGTGT252.3828437E-455.9999967
AAGGGCG252.3828437E-455.99999618
AAAAAGG13700.054.67153570
AATCCTG200.006937745552.518
TCCGCAT200.006937745552.520
CTTCATT200.006937745552.528
TTTCGCG200.006937745552.519
AACTTAA200.006937745552.528
Produced by FastQC (version 0.11.5)