FastQCFastQC Report
Wed 25 May 2016
SRR1780036_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780036_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125956
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1971015.648321636126902No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT1815014.409793896281242No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT92867.37241576423513No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC32162.5532725713741304No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG31762.5215154498396264No Hit
CACTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC11610.9217504525389818No Hit
CATAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT10180.8082187430531298No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT6630.5263742894344057No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC6570.52161072120423No Hit
CAAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT5560.4414239893296072No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT3400.26993553304328494RNA PCR Primer, Index 36 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3220.2556448283527581No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC2770.21991806662644098No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG1910.15164025532725714No Hit
CAAAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT1670.13258598240655467No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT1520.12067706183111562No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT1470.1167074216393026No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC1410.111943853409127No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCATT150.00222029570.0000114
AAGGTCC150.00222029570.0000138
ATAGAGA150.00222029570.000013
TAACGCA150.00222029570.0000111
TCTTTAT750.070.000011
AACTCCC150.00222029570.0000126
GCTAAAC150.00222029570.0000129
AGAGACG207.901282E-570.023
ATATACA11200.066.252
AGGGGGG35350.066.2376270
CATATAC11050.065.8823551
ACTTATA1500.063.0000042
CTTTATA850.061.7647062
AGCCGTC401.0570147E-861.2545
AAGCCGT401.0570147E-861.2544
TAAGCCG401.0570147E-861.2543
ACTCGTA352.9545845E-760.038
CAACTCG352.9545845E-760.036
CATAAAC1700.059.7058831
CACTTAT1600.059.06251
Produced by FastQC (version 0.11.5)