FastQCFastQC Report
Wed 25 May 2016
SRR1780035_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780035_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147954
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5283835.71245116725469No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG119248.059261662408586No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG13390.9050110169376969No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT12880.8705408437757682No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC11870.8022763832001838No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG3300.22304229693012692No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG2400.16221257958554686No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1960.13247360666152994No Hit
TATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGT1560.10543817673060545No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC207.9058416E-570.000013
CAGAACT150.002221064470.052
AACCGGG150.002221064470.042
GTCTGCC150.002221064470.056
CAGCCCG150.002221064470.016
TTCACAG252.8287686E-670.042
TACGGTC150.002221064470.010
CACCTGG150.002221064470.053
TCAATGC150.002221064470.015
GATGGCC150.002221064470.070
CTTGATT150.002221064470.022
CCCTTGA150.002221064470.020
TCTTTTC150.002221064470.053
GGATTCA150.002221064470.042
TATAAAG150.002221064470.04
ACGGTCA150.002221064470.011
ATGCTCT252.384211E-455.99999618
AAAAAGG11500.055.69565670
CTTGTCA200.0069401352.50000470
TGCCCTT200.0069401352.50000418
Produced by FastQC (version 0.11.5)