FastQCFastQC Report
Wed 25 May 2016
SRR1780035_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780035_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147954
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT1984413.412276788731633No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1937413.094610487043271No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT124028.382335050083134No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG41402.798166997850683No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC26581.7965043189099317No Hit
CATAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT11450.7738891817727132No Hit
CACTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC11070.7482055233383349No Hit
CAAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT7560.5109696256944727No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC7070.47785122402909014No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT6720.45419522283953123No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT6670.4508157940981657RNA PCR Primer, Index 36 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT3960.2676507563161523No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC3360.22709761141976562No Hit
AAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT2140.14463955013044597RNA PCR Primer, Index 36 (95% over 23bp)
CAAAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT2130.14396366438217284No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC1780.12030766319261392No Hit
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG1760.1189558916960677No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT1700.11490057720642903RNA PCR Primer, Index 36 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG1550.10476229098233235No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTGA150.002218640270.01926428
CGCTCAG150.002218640270.01926428
ACGCTCA150.002218640270.01926427
CGTCCCA150.002218640270.01926431
ACAAGTG150.002218640270.01926427
AACCGTC150.002221617669.99560543
AAACTCG600.069.99560536
CTCCGTT150.002221617669.99560552
TTAGCGA150.002221617669.99560513
CCATAGG150.002221617669.99560533
AAACCGT150.002221617669.99560542
TCGTCTT150.002221617669.99560546
CTGTTTA150.002221617669.99560513
CGAATGC550.069.99560541
TCCGTTT150.002221617669.99560553
CTTTCCC150.002221617669.99560533
AACTCGT550.069.99560537
ACTCCGT150.002221617669.99560551
GTCTTGC150.002221617669.99560548
TCCCAAC150.002221617669.99560533
Produced by FastQC (version 0.11.5)