FastQCFastQC Report
Wed 25 May 2016
SRR1780034_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780034_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170624
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4683727.450417291822955No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG96045.628750937734433No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG10880.6376594148537135No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC10380.6083552138034509No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT10220.5989778694673669No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG2890.16937828207051764No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG2090.12249156039009752No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAC150.002221463570.0014719
CACTCGT150.002221463570.0014718
TTGAGCG150.002224048669.980952
TGTATAA308.363148E-658.317462
CTGCAAC252.385001E-456.0011767
GAGCTCT200.006941365552.50110218
GAGAGCC200.006941365552.50110248
ATCCGCG200.006941365552.50110213
ATCCCGA200.006941365552.50110237
CGGGAGC200.006941365552.50110215
GCGCTTT200.006941365552.50110226
CATCCCG200.006941365552.50110236
TAAGCCA200.006941365552.50110215
ACGGTGA200.006941365552.50110229
ACTTGTC200.006949419652.4857143
CTATAGA200.006949419652.4857143
TGCGGGT200.006949419652.4857145
AAAAAGG11900.050.6041370
CTCGGTG16800.048.54268344
TCGGTGG16800.048.54268345
Produced by FastQC (version 0.11.5)