FastQCFastQC Report
Wed 25 May 2016
SRR1780034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170624
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT1746610.23654351087772Illumina PCR Primer Index 1 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158419.284156976744185No Hit
CATATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT103156.045456676669167Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG33401.9575206301575394No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC23791.3942938859714928No Hit
CATAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT8550.5011018379594899Illumina PCR Primer Index 1 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC8140.4770723930982746No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC7460.43721867966991745No Hit
CAAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT5410.31707145536384096Illumina PCR Primer Index 1 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT5200.3047636909227307Illumina PCR Primer Index 1 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT4990.2924559264816204Illumina PCR Primer Index 1 (95% over 24bp)
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT3280.19223555888972244Illumina PCR Primer Index 1 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC2610.1529679294823706Illumina PCR Primer Index 1 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT2350.13772974493623405TruSeq Adapter, Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT2120.12424981245311328Illumina PCR Primer Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCAT150.002219325270.0184764
ATGCCCA150.002219325270.0184763
TCTGAAA150.002221907769.9979533
GGAGCAA150.002221907769.9979545
ACGCCGC150.002221907769.9979541
CAGTTTC150.002221907769.9979556
CTATAAG150.002221907769.9979518
GCAAACG150.002221907769.9979518
CACGAAT150.002221907769.9979539
CTGAAAT150.002221907769.9979534
AATGCTC150.002221907769.9979528
TCAATGC150.002221907769.9979526
GGGAAAG150.002221907769.9979515
GTCAATG150.002221907769.9979525
CTCTGAA150.002221907769.9979532
GAACCTT150.002221907769.9979528
GCGCTCA150.002221907769.9979547
GACTCGC150.002221907769.9979544
TGACTCG150.002221907769.9979543
ATATACA13850.065.702042
Produced by FastQC (version 0.11.5)