FastQCFastQC Report
Wed 25 May 2016
SRR1780033_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780033_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334745
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4278412.781072159404921No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG100162.992128336494944No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG13530.404188262707434No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC12790.38208188322454406No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT10810.3229323813649196No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTA150.002223918469.99686462
GTCGCGT252.38911E-455.9974911
GGGTCTA252.38911E-455.997491
ATGGGTC200.00694898652.49764669
GCGTCAA200.00694898652.49764664
CGTTGTT305.874698E-446.66457756
TGTTCGT305.874698E-446.66457710
GGCCATT404.589009E-543.7480444
TAACCGG404.589009E-543.7480422
TTCGACA404.589009E-543.7480423
GCCGTAT16400.041.82739654
GTCGCCG16600.041.74511751
CGGTGGT16600.041.74511746
TGGTCGC16600.041.74511749
TCGCCGT16400.041.6139952
TCTCGGT16500.041.36178243
TCGGTGG16600.041.3234545
CTCGGTG16550.041.23682444
GTAGATC16800.041.03982538
AGATCTC16750.040.9533940
Produced by FastQC (version 0.11.5)