FastQCFastQC Report
Wed 25 May 2016
SRR1780033_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780033_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334745
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG169505.063555841013309No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT163964.898056729749511No Hit
CATATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT92862.774051890244813No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG31640.9451970903224842No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC27830.8313791094713887No Hit
CACTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC9460.2826031755515392No Hit
CATAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT8120.24257270459603578No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC6790.20284096849840927No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT5460.16310923240078268No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT5300.15832947467475245TruSeq Adapter, Index 25 (95% over 22bp)
CAAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT5220.15593959581173727No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTACG150.002219478170.0321422
GGTAGCC150.002224746669.9902945
AGGGGGG34000.060.32417370
AACTCGT352.9724106E-759.99167637
TAACTCG352.9724106E-759.99167636
AAGCCGT650.059.22255344
ATATACA11800.058.127532
CATATAC11550.057.870761
CGTATGA900.054.4368941
ACGTATG452.6988346E-854.4368940
CACGTAT452.6988346E-854.4368939
TGCCGTA653.6379788E-1253.83868445
CATGGAC200.00694745952.5005665
TGACGCG200.00695156652.4927239
GTAACTC200.00695156652.4927241
GCCGTAT608.367351E-1152.49271846
CGTCTTA552.2791937E-950.90963448
AGCCGTC707.2759576E-1249.99306545
AAGGGGG41000.049.93955669
TCGTATG42300.049.80396740