FastQCFastQC Report
Wed 25 May 2016
SRR1780032_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780032_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161827
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4622528.564454633651987No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG99856.170169378410278No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC15080.9318593312611615No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT10820.6686152496184197No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG10700.6611999233749621No Hit
TCTTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG3510.21689829262113244No Hit
CTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGG2850.1761139982821161No Hit
TATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGT1840.11370166906634863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAG150.002221012570.003437
TCCGTTG150.002221012570.003418
GGACTGA150.002223737869.9817666
CCCATAG150.002223737869.9817661
ATACATG150.002223737869.9817665
AAAAAGG11650.052.5776670
AAGGTAC200.006939963452.5025555
GATTCAC200.006939963452.5025530
ATCGAAA200.006939963452.5025518
CGCGTCT200.006948454352.486337
TGTACAC200.006948454352.486333
GCATGCT200.006948454352.486333
GCCGTAT17900.047.7118154
TCGCCGT17850.047.64937252
CGGTGGT18350.047.49549546
CGCCGTA18000.047.4467553
CTCGGTG18350.047.30475244
GATCTCG18300.047.2427341
CCGTATC18100.047.18461255
GTGGTCG18400.047.17620548
Produced by FastQC (version 0.11.5)