FastQCFastQC Report
Wed 25 May 2016
SRR1780032_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780032_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161827
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT1745410.785592021108963No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1712810.584142324828367No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT103256.380270288641573No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG31081.9205694970554976No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC28111.7370401725299238No Hit
CACTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC11250.6951868353241425No Hit
CATAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT9630.5950799310374659No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC7180.44368368690020826No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT6090.3763278068554691Illumina PCR Primer Index 6 (95% over 22bp)
CAAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT5740.354699771978718No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT5710.35284594041785367No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3250.20083175242697449No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC2660.16437306506330837No Hit
AAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT2100.12976820926050658Illumina PCR Primer Index 6 (95% over 22bp)
CAAAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT1740.10752223053013402No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC1620.10010690428667651No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCACG150.00221704770.0349420
CACGCTA150.00221704770.0349423
CTTACGT150.00221704770.0349424
ACAGTTG207.900638E-570.0132931
GCAAGTC150.00222249269.9916540
TACCATG150.00222249269.9916512
ACGGGGG150.00222249269.9916570
AACGGGG150.00222249269.9916569
GTGTCCC150.00222249269.991653
AGGGGGG38150.063.7538770
CATATAC12850.063.4554671
ATATACA13050.063.019312
AATCACG453.8198777E-1062.21480636
ATATCCG352.9623334E-759.99284410
CGTCTTA308.353629E-658.3263848
GTCTACT601.8189894E-1258.3263849
CTTTATA1150.057.8191952
ACGATCA800.056.9033927
CAGCCGA252.3790034E-456.0279521
GATGAAC252.3790034E-456.0279526
Produced by FastQC (version 0.11.5)