FastQCFastQC Report
Wed 25 May 2016
SRR1780031_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780031_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512857
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG432808.438999565180938No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG86061.6780506066993335No Hit
ATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTGGTC12050.23495828271818459No Hit
CTCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGG10220.19927582152529846No Hit
TCTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGT9950.19401119610339723No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTCG150.002224568369.997085
TAGTCGT150.002224568369.997086
ACCAGCG200.006948320752.50292616
CTTCGTT200.006951000552.4978128
TCGTTAG200.006951000552.4978130
GCTTCGT200.006951000552.4978127
GCGAGTT200.006951000552.4978166
TGGTCGC13250.048.0781549
CGGTGGT13350.047.980246
GCCGTAT13100.047.8224354
TCGCCGT13050.047.74212352
GTCGCCG13350.047.71801851
CTCGGTG13400.047.53996344
TCGGTGG13500.047.18781345
CGCGTCT305.8775506E-446.664727
ACTGGCG305.8775506E-446.664725
ATCTCGG13600.046.58347742
GATCTCG13600.046.58347741
AGATCTC13750.046.32985340
GTAGATC13750.046.32985338
Produced by FastQC (version 0.11.5)