FastQCFastQC Report
Wed 25 May 2016
SRR1780031_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780031_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512857
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT152552.974513363374196No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150082.9263517900701363No Hit
CATATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT86671.6899447604302953No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG27810.5422564184558268No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC22220.4332591736097976No Hit
CACTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC7870.15345408174208405No Hit
CATAAACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT6440.12557106561868123No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATG150.002224470369.99785658
CTAACGA150.002225330669.9910335
TGCCGAC401.3460522E-1069.9910345
AGGGGGG31200.060.120570
ATCGTAT509.822543E-1055.99282539
GCCGACT452.7024726E-854.4374746
AAACGTT200.006937303652.52399429
ATATCGT608.367351E-1152.49327537
CGAATGC608.367351E-1152.49327541
GGGTCTA200.006953376352.493271
AACGCGC200.006953376352.4932769
AACGAAG200.006953376352.4932737
CATATAC14150.052.4314461
CGTCTTA2350.052.1209848
AGCCGTC750.051.32675645
ATATACA14800.051.3109932
CGTATGC39250.051.17815441
GCCGTCT33400.051.13117246
TATGCCG38950.051.03325743
TCTCGTA39650.051.01489638