FastQCFastQC Report
Wed 25 May 2016
SRR1780030_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780030_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55750
Sequences flagged as poor quality0
Sequence length76
%GC85

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3713566.60986547085201No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG10011.7955156950672644No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1160.20807174887892377No Hit
TCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGT1150.20627802690582958No Hit
CTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGG1090.19551569506726457No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG920.16502242152466368No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGA150.002213786470.0000118
CTACAGG150.002213786470.000013
GAGAAGT150.002213786470.0000123
GGGTCCT150.002213786470.0000157
TCTAGTT150.002213786470.0000132
GGCACCA150.002213786470.0000118
GTCATAA150.002213786470.000012
AGCTTAG150.002213786470.0000142
AGTAGTC150.002213786470.0000127
GGTCCTG150.002213786470.0000158
ATGAATA150.002213786470.0000111
GGTCATA150.002213786470.000011
CTTAGGG150.002213786470.0000144
TAGTCTA150.002213786470.0000129
GATGAAT150.002213786470.0000110
AGGGAGA150.002213786470.0000147
TATCCGC150.002213786470.0000134
CTAGTTT150.002213786470.0000133
TGCTACA150.002213786470.000011
TTAGGGA150.002213786470.0000145
Produced by FastQC (version 0.11.5)