FastQCFastQC Report
Wed 25 May 2016
SRR1780030_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780030_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55750
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2323341.673542600896866No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG39797.1372197309417045No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC25834.633183856502242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17963.2215246636771298No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT9651.7309417040358746TruSeq Adapter, Index 11 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC8131.4582959641255606No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT4050.726457399103139No Hit
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC3510.6295964125560538No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT2650.47533632286995514No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT2540.4556053811659193No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC2150.38565022421524664No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT2110.37847533632286995RNA PCR Primer, Index 11 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1850.33183856502242154No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT1730.3103139013452915No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC1670.29955156950672646No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT1410.25291479820627805No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC1270.22780269058295965No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT1190.21345291479820627No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1170.20986547085201793No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAG1160.20807174887892377No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGA1080.1937219730941704No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG1080.1937219730941704No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCAT1020.18295964125560538No Hit
TCTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT730.13094170403587443No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT700.12556053811659193No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGAATGCCGT700.12556053811659193No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATATCGTATGCCGT670.12017937219730943No Hit
CTTATACACATCTCCGAGACCACGAGACTCAGCTATATCTCGTATGCCGT670.12017937219730943No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCA600.10762331838565023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT150.002213786470.0000139
CAAGAAA150.002213786470.0000157
AAACTAG150.002213786470.0000123
ATCTCGA150.002213786470.0000137
CTATAAC252.805671E-670.0000133
CCTGTAG150.002213786470.0000153
TAAGCAG150.002213786470.0000143
CCTAAGC150.002213786470.0000113
AGAGGGG150.002213786470.0000170
AAGAGGG150.002213786470.0000169
ATAACTC252.805671E-670.0000135
TAGCACT150.002213786470.0000157
TCACGTA150.002213786470.0000138
AAAAGAG150.002213786470.0000167
TCTCGAA150.002213786470.0000138
TAATTTC150.002213786470.0000162
GTCTTAA150.002213786470.0000149
TCTTGAA150.002213786470.0000156
TGTAGCA150.002213786470.0000155
GTAAGCA150.002213786470.0000142
Produced by FastQC (version 0.11.5)