##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780030_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55750 Sequences flagged as poor quality 0 Sequence length 76 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.084448430493275 32.0 27.0 32.0 22.0 32.0 2 30.728681614349775 32.0 32.0 32.0 27.0 32.0 3 30.985076233183857 32.0 32.0 32.0 32.0 32.0 4 30.675784753363228 32.0 32.0 32.0 27.0 32.0 5 31.01906726457399 32.0 32.0 32.0 27.0 32.0 6 33.32408968609865 37.0 32.0 37.0 22.0 37.0 7 35.30676233183856 37.0 37.0 37.0 32.0 37.0 8 32.93831390134529 37.0 32.0 37.0 22.0 37.0 9 35.11628699551569 37.0 37.0 37.0 32.0 37.0 10-11 35.11911210762332 37.0 37.0 37.0 29.5 37.0 12-13 35.48718385650224 37.0 37.0 37.0 32.0 37.0 14-15 35.31330941704036 37.0 37.0 37.0 32.0 37.0 16-17 33.98834080717489 37.0 34.5 37.0 24.5 37.0 18-19 34.244349775784755 37.0 37.0 37.0 27.0 37.0 20-21 35.20746188340807 37.0 37.0 37.0 32.0 37.0 22-23 34.5852466367713 37.0 37.0 37.0 29.5 37.0 24-25 34.97316591928251 37.0 37.0 37.0 32.0 37.0 26-27 35.09782959641255 37.0 37.0 37.0 29.5 37.0 28-29 35.46782959641256 37.0 37.0 37.0 32.0 37.0 30-31 35.197058295964126 37.0 37.0 37.0 29.5 37.0 32-33 35.164 37.0 37.0 37.0 32.0 37.0 34-35 34.50641255605382 37.0 34.5 37.0 29.5 37.0 36-37 34.627874439461884 37.0 37.0 37.0 27.0 37.0 38-39 34.57165919282511 37.0 37.0 37.0 27.0 37.0 40-41 33.670484304932735 37.0 34.5 37.0 22.0 37.0 42-43 33.12904035874439 37.0 34.5 37.0 17.5 37.0 44-45 33.849677130044846 37.0 34.5 37.0 24.5 37.0 46-47 32.93314798206278 37.0 32.0 37.0 17.5 37.0 48-49 31.19460089686099 37.0 29.5 37.0 10.5 37.0 50-51 32.512771300448435 37.0 32.0 37.0 13.0 37.0 52-53 31.592367713004485 37.0 29.5 37.0 17.5 37.0 54-55 31.9717399103139 37.0 29.5 37.0 13.0 37.0 56-57 30.74332735426009 37.0 27.0 37.0 13.0 37.0 58-59 31.676699551569506 37.0 29.5 37.0 13.0 37.0 60-61 31.81196412556054 37.0 29.5 37.0 17.5 37.0 62-63 34.053408071748876 37.0 34.5 37.0 27.0 37.0 64-65 34.463488789237665 37.0 37.0 37.0 27.0 37.0 66-67 34.40117488789238 37.0 37.0 37.0 27.0 37.0 68-69 33.856878923766814 37.0 34.5 37.0 24.5 37.0 70-71 30.420932735426007 34.5 22.5 37.0 17.5 37.0 72-73 31.30952466367713 37.0 29.5 37.0 13.0 37.0 74-75 33.121623318385645 37.0 32.0 37.0 13.0 37.0 76 33.53747085201794 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.12343407250326166 11101 2 -0.11143885187055957 11101 3 -0.16804860376560882 11101 4 -0.645824975309381 11101 5 -0.37666815723151004 11101 6 -0.04800977072665802 11101 7 0.550428015751045 11101 8 -0.1809436516463805 11101 9 0.26230762732600965 11101 10-11 0.39442958828706765 11101 12-13 -0.8389212533406649 11101 14-15 0.7352546222731746 11101 16-17 0.05081715531189701 11101 18-19 0.1580137880567989 11101 20-21 0.35282812655849227 11101 22-23 0.5221544663684838 11101 24-25 -0.2605492343592317 11101 26-27 0.3283716806607231 11101 28-29 -0.32587458335386543 11101 30-31 0.509363177231954 11101 32-33 1.2996300660270563 11101 34-35 1.1744548216207988 11101 36-37 1.1693841621076473 11101 38-39 0.41470188796593277 11101 40-41 -1.2867170470446112 11101 42-43 -0.8239799647493697 11101 44-45 -2.7087749832979426 11101 46-47 1.5676461228841632 11101 48-49 -2.758444714471274 11101 50-51 0.4398957894686859 11101 52-53 -1.5475025092919665 11101 54-55 -0.5537175068626361 11101 56-57 -1.4011568094283327 11101 58-59 -1.1391095969603242 11101 60-61 -0.9231047263365753 11101 62-63 -1.3959659002465727 11101 64-65 -0.06511892227821647 11101 66-67 -1.8554374150552775 11101 68-69 0.20740128416898074 11101 70-71 -1.9974399659233733 11101 72-73 -1.5207447090508666 11101 74-75 0.2933550763489805 11101 76 2.544516226776153 11102 1 1.083751532820724 11102 2 0.08300559257388329 11102 3 -1.1587893445063493 11102 4 0.1398893104049037 11102 5 -1.5393665699299248 11102 6 -0.5916605643774488 11102 7 -2.15988944456641 11102 8 1.0095325388298093 11102 9 0.99908011409849 11102 10-11 -1.2312317873743126 11102 12-13 -0.41233395175336085 11102 14-15 -0.8897453777268254 11102 16-17 -1.3956114161166724 11102 18-19 0.6957122007552172 11102 20-21 -1.1312988575684955 11102 22-23 -0.999009554795542 11102 24-25 -2.3213957952057953 11102 26-27 0.010911363200406754 11102 28-29 -0.4548428373221185 11102 30-31 -0.8557161878474133 11102 32-33 -0.9669043255073362 11102 34-35 -1.2792488820829035 11102 36-37 -2.074002081278593 11102 38-39 -3.227493879229833 11102 40-41 -1.5810292163567787 11102 42-43 -3.368292134061541 11102 44-45 -2.942901967424927 11102 46-47 -2.623491443253407 11102 48-49 -1.9469367779633373 11102 50-51 -1.1612946867217957 11102 52-53 -2.3484284352178904 11102 54-55 -1.619193697338826 11102 56-57 -0.5539345872061112 11102 58-59 -0.8348767927275205 11102 60-61 -1.4654327686646162 11102 62-63 -0.5408071700878452 11102 64-65 -0.30718241434171034 11102 66-67 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11103 46-47 -2.0600677979332076 11103 48-49 0.06722578361301856 11103 50-51 0.07207969751465981 11103 52-53 0.785192254437284 11103 54-55 -1.3698094608856266 11103 56-57 -0.8685897596199048 11103 58-59 -2.361970388786631 11103 60-61 0.581365260892035 11103 62-63 -0.9342800764917811 11103 64-65 -0.8256553207456463 11103 66-67 -0.07318965004888867 11103 68-69 -0.9318068895219511 11103 70-71 -3.0983339633690967 11103 72-73 -2.041178936381648 11103 74-75 -0.6300165711605885 11103 76 0.2635456009779418 11104 1 -1.682121483052292 11104 2 0.3885611481294404 11104 3 -0.51990045561746 11104 4 -0.5347138641982703 11104 5 -0.1544459350092886 11104 6 0.20199022927334198 11104 7 -0.7829053175822835 11104 8 -3.4587214294241555 11104 9 -0.1358405208221427 11104 10-11 -0.1657555968981228 11104 12-13 -1.213921253340665 11104 14-15 -1.2091898221712682 11104 16-17 -0.7825161780214316 11104 18-19 -0.3697639897209726 11104 20-21 -0.5360607623304006 11104 22-23 -1.22321590400189 11104 24-25 0.31352483971483736 11104 26-27 -0.727183874894834 11104 28-29 0.5352365277572417 11104 30-31 -0.5323034894347103 11104 32-33 -1.1494440080470198 11104 34-35 -0.19591554874957495 11104 36-37 -0.534319541596048 11104 38-39 -0.4140018157377767 11104 40-41 -2.374680010007573 11104 42-43 -2.3563873721567745 11104 44-45 -1.6532194277423855 11104 46-47 -1.3258723956343559 11104 48-49 -2.3695558255823883 11104 50-51 -1.948993099420207 11104 52-53 -0.7650951018845582 11104 54-55 -1.3870508401959682 11104 56-57 -2.623379031650554 11104 58-59 -3.3011466339973623 11104 60-61 0.35004342181157355 11104 62-63 -0.8126325669132441 11104 64-65 0.26821441105511923 11104 66-67 -0.7350670446849037 11104 68-69 0.4111049878726831 11104 70-71 -0.36318070666411373 11104 72-73 -1.6225965609027213 11104 74-75 -0.9566449236510195 11104 76 -4.029557847297923 11105 1 1.1474580965272878 11105 2 -0.12645386688557636 11105 3 0.30742687170986827 11105 4 0.029850700366296223 11105 5 0.5272357466723925 11105 6 -1.1621238848407742 11105 7 0.8507283160513452 11105 8 0.20644373574101138 11105 9 0.027072392090772723 11105 10-11 0.2137489076063872 11105 12-13 -0.11069302511243251 11105 14-15 0.3839032709218202 11105 16-17 -0.05729095279620822 11105 18-19 0.6730288030718157 11105 20-21 0.7297050034353703 11105 22-23 0.30794025215426046 11105 24-25 0.9816930078830097 11105 26-27 0.12867198096103039 11105 28-29 0.2386899812106975 11105 30-31 0.26011392798270094 11105 32-33 1.1069373733343681 11105 34-35 -0.5022218550558719 11105 36-37 0.6248396175631115 11105 38-39 -0.20191472865067794 11105 40-41 -0.41934967967724646 11105 42-43 0.6514955107261073 11105 44-45 0.05999378547082301 11105 46-47 -0.4598814046433688 11105 48-49 0.7475612915347298 11105 50-51 0.3633192128921081 11105 52-53 -0.23018519197464826 11105 54-55 -1.332996786141912 11105 56-57 -0.3140697223412481 11105 58-59 0.9199494620987352 11105 60-61 -0.6758574790893235 11105 62-63 0.3457758414951613 11105 64-65 -0.3293831865424792 11105 66-67 0.8317497721319143 11105 68-69 1.2023962791639775 11105 70-71 -1.1766191451025527 11105 72-73 -1.4116355999417607 11105 74-75 0.5666283496222562 11105 76 1.2001718824318033 11106 1 -2.0666244070289004 11106 2 0.2803740136265134 11106 3 -0.19826302871687673 11106 4 1.0910171299537765 11106 5 -0.09011844962917337 11106 6 -2.691284624527828 11106 7 -1.5753029784009982 11106 8 -0.3476103183130448 11106 9 0.8393056780082659 11106 10-11 0.191700543452761 11106 12-13 -0.46026628258043445 11106 14-15 -0.5936927461478803 11106 16-17 -0.2649723183723083 11106 18-19 0.5688325015070959 11106 20-21 -0.7948326921549622 11106 22-23 -0.26780654727674147 11106 24-25 -1.1089897801682014 11106 26-27 0.38685121282446744 11106 28-29 -0.0685646418334045 11106 30-31 -0.5286485186744727 11106 32-33 -0.6238592127253781 11106 34-35 -1.792406776819746 11106 36-37 -0.29016749481242243 11106 38-39 0.9332204064844518 11106 40-41 0.03833168589885361 11106 42-43 -0.24893123180590493 11106 44-45 -0.3038042230640272 11106 46-47 1.3210574289270482 11106 48-49 0.7927248761719987 11106 50-51 0.0012992982406103692 11106 52-53 0.8292031437294796 11106 54-55 2.1392649492777167 11106 56-57 0.5447496233202038 11106 58-59 -0.6995384468628565 11106 60-61 -0.8319741220480736 11106 62-63 -0.5699425084337051 11106 64-65 -0.4350019632138853 11106 66-67 -0.7496869277258398 11106 68-69 0.28098802880835194 11106 70-71 -0.46040292888633516 11106 72-73 0.4461168893896783 11106 74-75 -1.2929022335925353 11106 76 -0.7663999525610805 11107 1 -0.13473586213726207 11107 2 0.4506526513974123 11107 3 0.6500341849054152 11107 4 -0.9399426223682035 11107 5 0.06777628721293283 11107 6 -0.8878790517724084 11107 7 -1.1914020496084277 11107 8 -1.1417279653718673 11107 9 0.15664313930857787 11107 10-11 0.4935581285920776 11107 12-13 -0.4238885736021061 11107 14-15 0.49996050462611663 11107 16-17 -1.346241668217516 11107 18-19 0.198863461259414 11107 20-21 -1.2909627231147098 11107 22-23 -0.4380851850476404 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6 -2.0130891358060232 11108 7 1.6615391268621593 11108 8 -0.19046746117018643 11108 9 -0.6223484573300766 11108 10-11 0.9616253554828376 11108 12-13 0.605523191103778 11108 14-15 -0.5790310920125421 11108 16-17 0.07938858388332193 11108 18-19 0.8707122007552144 11108 20-21 0.4544154281457935 11108 22-23 1.1581333023473164 11108 24-25 0.7643184905084865 11108 26-27 -0.3176600653710153 11108 28-29 0.16301430553502172 11108 30-31 0.1014266692954493 11108 32-33 -0.46333289693590274 11108 34-35 0.2850368322028132 11108 36-37 0.24028363300712385 11108 38-39 0.018934692198740777 11108 40-41 1.3582564979289344 11108 42-43 1.7495650087956065 11108 44-45 -0.6786162531392108 11108 46-47 1.2729371281751654 11108 48-49 0.19234893632237515 11108 50-51 0.14941959899249468 11108 52-53 1.183714421924968 11108 54-55 -2.4013365544816807 11108 56-57 0.8067796985081763 11108 58-59 0.13655177870105462 11108 60-61 0.2774243741925275 11108 62-63 0.6341928299121591 11108 64-65 -0.08575384291314236 11108 66-67 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21612 9 0.4529128667117348 21612 10-11 -0.5021377107192535 21612 12-13 -0.2481353454815931 21612 14-15 0.16208389056585304 21612 16-17 0.1727683748240949 21612 18-19 0.4452766606855292 21612 20-21 0.6509311075882991 21612 22-23 0.2100496786539381 21612 24-25 0.18208852535168774 21612 26-27 0.2077754746986642 21612 28-29 -0.8028393530015663 21612 30-31 0.27494583305850995 21612 32-33 -0.35845484815541795 21612 34-35 -0.5292488820829035 21612 36-37 -0.3771379697803354 21612 38-39 -0.8472673983935977 21612 40-41 1.9115666024585494 21612 42-43 1.1791817335342785 21612 44-45 -1.1057939534876482 21612 46-47 -0.052498411894518426 21612 48-49 1.0003628736046082 21612 50-51 -0.28368144630367453 21612 52-53 2.075352052586986 21612 54-55 0.8080711110235441 21612 56-57 -0.581373611596355 21612 58-59 0.7330674581435588 21612 60-61 -0.6692655212778575 21612 62-63 -0.7320092606801794 21612 64-65 -0.025823529324284777 21612 66-67 -1.3360155541699967 21612 68-69 0.14010227784558538 21612 70-71 1.5517921930648875 21612 72-73 0.6951541166040585 21612 74-75 -0.0833386905886826 21612 76 -0.9198017497369477 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 2.0 18 1.0 19 12.0 20 21.0 21 34.0 22 73.0 23 130.0 24 266.0 25 383.0 26 647.0 27 912.0 28 1305.0 29 1794.0 30 2657.0 31 3753.0 32 5407.0 33 8238.0 34 12490.0 35 13858.0 36 3766.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.829763570480392 9.471531589710471 14.829763570480392 60.86894126932874 2 9.060414723398148 6.647772117385377 66.15304584917845 18.138767310038027 3 8.753363228699552 10.588340807174887 68.36053811659193 12.297757847533632 4 7.203587443946188 55.05650224215247 27.17488789237668 10.565022421524665 5 7.558744394618834 22.8 63.865470852017935 5.7757847533632285 6 11.013650469066711 66.63079158370554 14.348239430303684 8.007318516924071 7 10.825112107623317 21.216143497757848 12.995515695067263 54.96322869955157 8 9.528251121076234 66.33363228699551 7.865470852017937 16.272645739910313 9 9.562331838565022 16.127354260089685 5.583856502242153 68.72645739910314 10-11 9.969506726457398 40.01345291479821 33.23497757847534 16.782062780269058 12-13 11.303139013452915 9.85829596412556 40.59551569506726 38.243049327354264 14-15 11.74170403587444 7.658295964125561 14.25560538116592 66.34439461883407 16-17 40.2 32.05201793721973 6.054708520179372 21.693273542600895 18-19 43.210762331838566 16.442152466367713 6.32914798206278 34.01793721973094 20-21 14.976681614349777 7.76591928251121 5.982062780269058 71.27533632286995 22-23 11.71121076233184 37.82959641255606 5.921973094170404 44.5372197309417 24-25 40.44020700851175 37.01936444440458 6.108903697990009 16.431524849093666 26-27 43.01808266360505 40.46085677382319 7.824160447761194 8.696900114810562 28-29 15.025609285720693 16.438380739664343 31.70887041073527 36.8271395638797 30-31 10.355467453604456 32.19926987002969 16.21803438965978 41.22722828670607 32-33 38.90558151632838 16.864735902702414 8.711757688823514 35.5179248921457 34-35 13.241493120952844 33.623921505318485 38.206066476528726 14.92851889719995 36-37 9.414349775784753 40.99461883408072 41.63408071748879 7.956950672645739 38-39 10.286995515695068 15.31031390134529 41.220627802690586 33.18206278026906 40-41 10.209865470852018 7.926457399103139 41.67443946188341 40.18923766816144 42-43 39.77578475336323 7.0789237668161435 37.691479820627805 15.453811659192827 44-45 13.805381165919284 35.81076233183856 41.11300448430493 9.27085201793722 46-47 39.39372197309417 10.58744394618834 17.56053811659193 32.45829596412556 48-49 37.572197309417035 7.026905829596412 9.820627802690582 45.58026905829596 50-51 16.77488789237668 7.338116591928251 39.184753363228694 36.70224215246637 52-53 8.965022421524663 8.72645739910314 66.33004484304934 15.97847533632287 54-55 8.266738418763172 8.694560294183596 45.20113009551998 37.83757119153325 56-57 38.06008968609865 7.595515695067265 18.10224215246637 36.24215246636771 58-59 12.95215032064218 11.059688775281403 60.25830754742365 15.729853356652765 60-61 36.97040358744395 36.281614349775786 19.173991031390134 7.573991031390134 62-63 11.667264573991032 73.62242152466368 6.481614349775784 8.228699551569507 64-65 7.955442347707541 78.14540431943747 5.696168472411566 8.202984860443424 66-67 8.252091947012978 78.1298486981946 5.748930483681468 7.869128871110951 68-69 10.770403587443948 75.82242152466367 5.510313901345292 7.896860986547086 70-71 33.60807174887893 52.60358744394619 5.62780269058296 8.160538116591928 72-73 75.43318385650224 11.087892376681614 5.401793721973094 8.077130044843049 74-75 80.38299399049242 6.425688402547314 5.197775585254282 7.9935420217059825 76 80.41793721973094 6.172197309417041 5.779372197309416 7.6304932735426005 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 5.0 16 6.0 17 3.0 18 8.0 19 12.5 20 12.0 21 12.0 22 18.0 23 30.0 24 56.0 25 76.0 26 75.5 27 106.5 28 147.0 29 156.0 30 142.0 31 151.0 32 200.0 33 226.0 34 234.5 35 249.5 36 334.5 37 413.0 38 436.5 39 584.0 40 764.0 41 944.0 42 1068.0 43 1277.5 44 2505.0 45 7793.0 46 12063.0 47 17064.5 48 11863.5 49 1644.5 50 1628.0 51 1136.0 52 566.5 53 502.5 54 516.0 55 532.0 56 550.0 57 604.5 58 657.0 59 565.5 60 430.5 61 374.0 62 361.0 63 371.0 64 313.0 65 247.5 66 218.0 67 186.0 68 168.5 69 140.5 70 126.5 71 123.0 72 110.0 73 77.5 74 44.5 75 31.0 76 26.5 77 22.5 78 17.5 79 12.0 80 18.0 81 21.5 82 10.0 83 1.0 84 3.5 85 3.5 86 1.5 87 2.0 88 2.0 89 1.5 90 0.5 91 1.0 92 2.0 93 2.5 94 6.0 95 10.0 96 11.0 97 19.5 98 61.5 99 912.5 100 1730.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007174887892376682 2 0.003587443946188341 3 0.0 4 0.0 5 0.0 6 0.0017937219730941704 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006278026905829597 26-27 0.010762331838565023 28-29 0.01524663677130045 30-31 0.011659192825112108 32-33 0.006278026905829597 34-35 0.0017937219730941704 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.004484304932735426 56-57 0.0 58-59 0.004484304932735426 60-61 0.0 62-63 0.0 64-65 0.003587443946188341 66-67 8.968609865470852E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.008968609865470852 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 55750.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 14.513004484304934 #Duplication Level Percentage of deduplicated Percentage of total 1 67.02508960573476 9.727354260089687 2 7.872945247806204 2.2852017937219733 3 5.685329378321592 2.475336322869955 4 4.498825855889259 2.611659192825112 5 4.128043505129156 2.9955156950672643 6 3.0527746879248547 2.6582959641255606 7 2.3359288097886544 2.3730941704035873 8 1.903349400568533 2.209865470852018 9 1.0258311704362872 1.3399103139013453 >10 2.1010999876405885 4.970403587443946 >50 0.08651588184402423 0.8233183856502242 >100 0.2101099987640588 5.675336322869955 >500 0.024718823384006924 3.189237668161435 >1k 0.03707823507601038 14.991928251121076 >5k 0.0 0.0 >10k+ 0.012359411692003462 41.673542600896866 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT 23233 41.673542600896866 No Hit TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG 3979 7.1372197309417045 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC 2583 4.633183856502242 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 1796 3.2215246636771298 No Hit ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT 965 1.7309417040358746 TruSeq Adapter, Index 11 (95% over 22bp) TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC 813 1.4582959641255606 No Hit ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT 405 0.726457399103139 No Hit CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC 351 0.6295964125560538 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT 265 0.47533632286995514 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT 254 0.4556053811659193 No Hit TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC 215 0.38565022421524664 No Hit TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT 211 0.37847533632286995 RNA PCR Primer, Index 11 (95% over 21bp) CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG 185 0.33183856502242154 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT 173 0.3103139013452915 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC 167 0.29955156950672646 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT 141 0.25291479820627805 No Hit CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC 127 0.22780269058295965 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT 119 0.21345291479820627 No Hit CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG 117 0.20986547085201793 No Hit TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAG 116 0.20807174887892377 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGA 108 0.1937219730941704 No Hit CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG 108 0.1937219730941704 No Hit ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCAT 102 0.18295964125560538 No Hit TCTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT 73 0.13094170403587443 No Hit CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT 70 0.12556053811659193 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGAATGCCGT 70 0.12556053811659193 No Hit CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATATCGTATGCCGT 67 0.12017937219730943 No Hit CTTATACACATCTCCGAGACCACGAGACTCAGCTATATCTCGTATGCCGT 67 0.12017937219730943 No Hit TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCA 60 0.10762331838565023 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0017937219730941704 0.0 2 0.0 0.0 0.0 0.0017937219730941704 0.0 3 0.0 0.0 0.0 0.0017937219730941704 0.0 4 0.0 0.0 0.0 0.0017937219730941704 0.0 5 0.0 0.0 0.0 0.021524663677130046 0.0 6 0.0 0.0 0.0 0.021524663677130046 0.0 7 0.0 0.0 0.0 0.021524663677130046 0.0 8 0.0 0.0 0.0 0.021524663677130046 0.0 9 0.0 0.0 0.0 0.021524663677130046 0.0 10 0.0 0.0 0.0 0.028699551569506727 0.0 11 0.0 0.0 0.0 0.028699551569506727 0.0 12 0.0 0.0 0.0 0.028699551569506727 0.0 13 0.0 0.0 0.0 0.028699551569506727 0.0 14 0.0 0.0 0.0 0.039461883408071746 0.0 15 0.0 0.0 0.0 0.039461883408071746 0.0 16 0.0 0.0 0.0 0.04663677130044843 0.0 17 0.0 0.0 0.0 0.05560538116591928 0.0 18 0.0 0.0 0.0 0.05739910313901345 0.0 19 0.0 0.0 0.0 0.07892376681614349 0.0 20 0.0 0.0 0.0 0.09506726457399103 0.0 21 0.0 0.0 0.0 0.12017937219730941 0.0 22 0.0 0.0 0.0 0.12017937219730941 0.0 23 0.0 0.0 0.0 0.13273542600896862 0.0 24 0.0 0.0 0.0 0.13811659192825113 0.0 25 0.0 0.0 0.0 0.2116591928251121 0.0 26 0.0 0.0 0.0 0.21345291479820627 0.0 27 0.0 0.0 0.0 0.21883408071748878 0.0 28 0.0 0.0 0.0 0.23318385650224216 0.0 29 0.0 0.0 0.0 0.25291479820627805 0.0 30 0.0 0.0 0.0 0.28340807174887894 0.0 31 0.0 0.0 0.0 0.31928251121076234 0.0 32 0.0 0.0 0.0 0.39461883408071746 0.0 33 0.0 0.0 0.0 0.47533632286995514 0.0 34 0.0 0.0 0.0 0.5596412556053811 0.0 35 0.0 0.0 0.0 0.6798206278026906 0.0 36 0.0 0.0 0.0 0.9542600896860987 0.0 37 0.0 0.0 0.0 1.4582959641255606 0.0 38 0.0 0.0 0.0 1.7955156950672646 0.0 39 0.0 0.0 0.0 2.2690582959641254 0.0 40 0.0 0.0 0.0 2.6116591928251123 0.0 41 0.0 0.0 0.0 2.977578475336323 0.0 42 0.0 0.0 0.0 3.5300448430493274 0.0 43 0.0 0.0 0.0 4.12914798206278 0.0 44 0.0 0.0 0.0 4.59372197309417 0.0 45 0.0 0.0 0.0 5.280717488789238 0.0 46 0.0 0.0 0.0 5.714798206278027 0.0 47 0.0 0.0 0.0 6.136322869955157 0.0 48 0.0 0.0 0.0 6.439461883408072 0.0 49 0.0 0.0 0.0 6.95067264573991 0.0 50 0.0 0.0 0.0 7.381165919282512 0.0 51 0.0 0.0 0.0 7.714798206278027 0.0 52 0.0 0.0 0.0 8.152466367713005 0.0 53 0.0 0.0 0.0 8.468161434977578 0.0 54 0.0 0.0 0.0 8.767713004484305 0.0 55 0.0 0.0 0.0 9.103139013452914 0.0 56 0.0 0.0 0.0 9.486995515695067 0.0 57 0.0 0.0 0.0 9.72017937219731 0.0 58 0.0 0.0 0.0 10.02152466367713 0.0 59 0.0 0.0 0.0 10.243946188340807 0.0 60 0.0 0.0 0.0 10.554260089686098 0.0 61 0.0 0.0 0.0 10.796412556053811 0.0 62 0.0 0.0 0.0 11.009865470852018 0.0 63 0.0 0.0 0.0 11.223318385650224 0.0 64 0.0 0.0 0.0 11.443946188340806 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGAAT 15 0.0022137864 70.00001 39 CAAGAAA 15 0.0022137864 70.00001 57 AAACTAG 15 0.0022137864 70.00001 23 ATCTCGA 15 0.0022137864 70.00001 37 CTATAAC 25 2.805671E-6 70.00001 33 CCTGTAG 15 0.0022137864 70.00001 53 TAAGCAG 15 0.0022137864 70.00001 43 CCTAAGC 15 0.0022137864 70.00001 13 AGAGGGG 15 0.0022137864 70.00001 70 AAGAGGG 15 0.0022137864 70.00001 69 ATAACTC 25 2.805671E-6 70.00001 35 TAGCACT 15 0.0022137864 70.00001 57 TCACGTA 15 0.0022137864 70.00001 38 AAAAGAG 15 0.0022137864 70.00001 67 TCTCGAA 15 0.0022137864 70.00001 38 TAATTTC 15 0.0022137864 70.00001 62 GTCTTAA 15 0.0022137864 70.00001 49 TCTTGAA 15 0.0022137864 70.00001 56 TGTAGCA 15 0.0022137864 70.00001 55 GTAAGCA 15 0.0022137864 70.00001 42 >>END_MODULE