FastQCFastQC Report
Wed 25 May 2016
SRR1780029_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780029_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70874
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3432148.42537460846009No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG8561.2077771820413692No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1040.1467392837994187No Hit
CTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGG980.13827355588791376No Hit
TCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGT970.13686260123599628No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750.10582159889381156No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAATT150.002211308670.0395418
CCCAAAG150.00221751569.9901251
GCTAGTG150.00221751569.9901265
CTTGTGC252.37736E-455.9920961
AGGGCAT200.00690980752.5296629
CCTGACT200.00690980752.5296631
AAGTACA200.006929144752.4925889
CGAAAGC200.006929144752.49258850
ACTCTTT200.006929144752.49258811
ACACTCT200.006929144752.4925889
GGTCTCT200.006929144752.49258858
GCTAACA200.006929144752.49258824
TGTGCGC305.8459886E-446.660083
GTCGCCG2000.045.49357651
TCTCGGT1850.045.39899443
TACGTGT2100.045.02542533
ACGTGTA2100.044.99365234
ATCTCGG1950.044.8654642
CTCGGTG1950.044.8654644
TCGGTGG1950.044.8654645
Produced by FastQC (version 0.11.5)