##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780029_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70874 Sequences flagged as poor quality 0 Sequence length 76 %GC 75 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.027824025735814 32.0 27.0 32.0 22.0 32.0 2 29.673505093546293 32.0 32.0 32.0 22.0 32.0 3 29.47242994610153 32.0 32.0 32.0 22.0 32.0 4 29.02194034483732 32.0 32.0 32.0 13.0 32.0 5 29.774219036600165 32.0 32.0 32.0 22.0 32.0 6 33.89423483929227 37.0 37.0 37.0 22.0 37.0 7 33.65255241696532 37.0 37.0 37.0 22.0 37.0 8 33.89937071422524 37.0 37.0 37.0 27.0 37.0 9 34.006179981375396 37.0 37.0 37.0 27.0 37.0 10-11 33.85877754888958 37.0 37.0 37.0 22.0 37.0 12-13 33.67616474306516 37.0 37.0 37.0 22.0 37.0 14-15 34.32262183593419 37.0 37.0 37.0 27.0 37.0 16-17 34.33153201456105 37.0 37.0 37.0 27.0 37.0 18-19 33.87264017834467 37.0 37.0 37.0 24.5 37.0 20-21 34.27247650760505 37.0 37.0 37.0 24.5 37.0 22-23 34.30233936281288 37.0 37.0 37.0 24.5 37.0 24-25 33.85221660975816 37.0 37.0 37.0 22.0 37.0 26-27 34.193336061179 37.0 37.0 37.0 24.5 37.0 28-29 34.128502694923384 37.0 37.0 37.0 27.0 37.0 30-31 34.086025905127414 37.0 37.0 37.0 27.0 37.0 32-33 34.08964500380958 37.0 37.0 37.0 27.0 37.0 34-35 34.11550074780597 37.0 37.0 37.0 27.0 37.0 36-37 34.6252010610379 37.0 37.0 37.0 29.5 37.0 38-39 34.700807066060904 37.0 37.0 37.0 29.5 37.0 40-41 34.283524282529555 37.0 37.0 37.0 27.0 37.0 42-43 34.24221153032141 37.0 37.0 37.0 27.0 37.0 44-45 34.037531393741006 37.0 37.0 37.0 24.5 37.0 46-47 34.174415159296785 37.0 37.0 37.0 24.5 37.0 48-49 34.60662584304541 37.0 37.0 37.0 29.5 37.0 50-51 34.84993791799532 37.0 37.0 37.0 32.0 37.0 52-53 34.16444676468098 37.0 37.0 37.0 27.0 37.0 54-55 34.287848858537686 37.0 37.0 37.0 27.0 37.0 56-57 34.0889254169371 37.0 37.0 37.0 24.5 37.0 58-59 33.81323898749894 37.0 37.0 37.0 22.0 37.0 60-61 33.66777661765951 37.0 37.0 37.0 22.0 37.0 62-63 33.369458475604596 37.0 37.0 37.0 22.0 37.0 64-65 33.199840562124336 37.0 37.0 37.0 17.5 37.0 66-67 33.26446933995541 37.0 37.0 37.0 22.0 37.0 68-69 33.40390693343116 37.0 37.0 37.0 22.0 37.0 70-71 33.43872929424049 37.0 37.0 37.0 22.0 37.0 72-73 33.70199226796851 37.0 37.0 37.0 22.0 37.0 74-75 33.834403307277704 37.0 37.0 37.0 22.0 37.0 76 33.742924062420634 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.08833617450948239 11101 2 -0.2368525092954421 11101 3 1.2840533324020882 11101 4 0.510068110128632 11101 5 -0.33363979292679247 11101 6 -0.6243407450629803 11101 7 1.0240099025291869 11101 8 1.0512070242217675 11101 9 2.130859640304166 11101 10-11 1.626861495910461 11101 12-13 1.5865453818577109 11101 14-15 1.121837321685618 11101 16-17 0.5838187828205648 11101 18-19 1.1209165837561557 11101 20-21 -1.9979703761973369 11101 22-23 0.4100619363083169 11101 24-25 1.6609916933069258 11101 26-27 0.2558427658299536 11101 28-29 0.5523535443000185 11101 30-31 -0.3692372333089011 11101 32-33 1.5682006091654728 11101 34-35 -1.769406180379363 11101 36-37 -0.9224859729417005 11101 38-39 0.34761626523231115 11101 40-41 -0.8476881527474802 11101 42-43 -0.8732754963356726 11101 44-45 0.5723369437597725 11101 46-47 0.48331146119809887 11101 48-49 0.5997163500799303 11101 50-51 0.12097911592864818 11101 52-53 0.5321324843087183 11101 54-55 -0.012681364425219499 11101 56-57 0.6712355254435778 11101 58-59 0.14727379877884772 11101 60-61 0.03132459705107493 11101 62-63 -0.173890199568433 11101 64-65 0.5695090139309471 11101 66-67 1.3622612485812908 11101 68-69 1.6277959718675419 11101 70-71 -0.6869707742477047 11101 72-73 1.2452670001491981 11101 74-75 0.7554692060782457 11101 76 -0.9130964125410657 11102 1 -0.07457673840343304 11102 2 -0.27213779458072906 11102 3 -1.1423730940243395 11102 4 -1.2038958538353342 11102 5 -1.9372433965303948 11102 6 -0.6258422465644813 11102 7 -0.5855997070804264 11102 8 0.5729787959935422 11102 9 -1.352623843179316 11102 10-11 0.1152248592738232 11102 12-13 0.32490874522107305 11102 14-15 1.4221376219859252 11102 16-17 0.694554518556302 11102 18-19 0.9891598269993978 11102 20-21 -1.1507481539751154 11102 22-23 -0.054652778406399705 11102 24-25 -0.1209152135999858 11102 26-27 1.4874493724365578 11102 28-29 -0.3703191283726497 11102 30-31 -1.1173603564320231 11102 32-33 -1.2854029944381367 11102 34-35 -0.398159934133119 11102 36-37 0.09966117420545118 11102 38-39 0.2811748237908702 11102 40-41 -0.033123588182917274 11102 42-43 -0.1259031239632975 11102 44-45 0.17669129811412887 11102 46-47 -1.6705924427058036 11102 48-49 -0.134517884154306 11102 50-51 0.513997133946674 11102 52-53 0.19279314496937872 11102 54-55 -0.8163600431038986 11102 56-57 -0.23754825834020465 11102 58-59 0.06731884382389097 11102 60-61 -0.0681498774234015 11102 62-63 -2.7054217310999604 11102 64-65 -0.7195300251080923 11102 66-67 -1.5056066192865813 11102 68-69 -0.9806875116159404 11102 70-71 -0.6359197231966576 11102 72-73 -1.5509041710219762 11102 74-75 -1.0298160792070377 11102 76 0.47053722109256313 11103 1 -0.4760269691219534 11103 2 0.18929925222217037 11103 3 -2.0547000551317893 11103 4 -1.6139156297087673 11103 5 -0.008436540894269484 11103 6 -1.4359938615941488 11103 7 -2.1900822383922254 11103 8 0.015299165143176197 11103 9 -1.953151199804232 11103 10-11 -1.7061330840353435 11103 12-13 -0.45986383223443283 11103 14-15 -0.8768076647642431 11103 16-17 -0.6604224095913622 11103 18-19 -1.3150590259999433 11103 20-21 -1.7117237637312144 11103 22-23 -0.016089825209299136 11103 24-25 -1.9768809354193628 11103 26-27 -1.394224984847554 11103 28-29 -0.9523890031254467 11103 30-31 -0.8424757156937304 11103 32-33 -0.3809863827044424 11103 34-35 -0.9479292156097188 11103 36-37 -1.2737732358143319 11103 38-39 -0.7082780437107701 11103 40-41 -0.23318950776103264 11103 42-43 -1.074156255143265 11103 44-45 -1.3470397500071627 11103 46-47 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1.0713452286604621 11104 26-27 -0.5042582442710568 11104 28-29 -0.22289898095250749 11104 30-31 -0.004338243409911513 11104 32-33 0.7121400031048637 11104 34-35 -0.03329506926825587 11104 36-37 -0.7053142557699843 11104 38-39 -0.32662615901011094 11104 40-41 0.450291645232312 11104 42-43 -1.2659522640124408 11104 44-45 -0.3392792178563795 11104 46-47 -0.06340571051907062 11104 48-49 -1.1010412256776476 11104 50-51 0.27628214623167935 11104 52-53 -0.6143321621559323 11104 54-55 -0.39651974826360714 11104 56-57 0.15987188907993755 11104 58-59 -1.0156049890999412 11104 60-61 -0.024230958504482203 11104 62-63 -0.3178295935078239 11104 64-65 0.09097366039559773 11104 66-67 0.046604682924723306 11104 68-69 -0.3065474624758906 11104 70-71 -0.9003546126315456 11104 72-73 -0.36079360591141096 11104 74-75 1.040822741431782 11104 76 1.394984395539744 11105 1 0.6759430121163206 11105 2 -0.49112601356894814 11105 3 -0.46808341973466483 11105 4 -0.8809575308970103 11105 5 1.0766166173296199 11105 6 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66-67 0.8280732143932568 11105 68-69 1.9215993906709627 11105 70-71 0.44550785823092554 11105 72-73 0.4792413591235558 11105 74-75 -0.10777865716961799 11105 76 1.7685275190828662 11106 1 -1.1894416032682962 11106 2 -1.959074731517667 11106 3 -0.41039111204235823 11106 4 -1.031598556538036 11106 5 -1.4794731262601246 11106 6 -1.4507296339518732 11106 7 -1.9065456530263702 11106 8 -3.4349040868893432 11106 9 -1.7163625819180552 11106 10-11 -1.6266107263117604 11106 12-13 -1.1460935070311749 11106 14-15 -1.6212182338699392 11106 16-17 -0.7356256616238852 11106 18-19 -1.3790834162438443 11106 20-21 -1.5674148206417797 11106 22-23 -0.7114658414694617 11106 24-25 -2.0508138622486314 11106 26-27 -1.9872127897256036 11106 28-29 -0.36778534458886725 11106 30-31 -1.664376122197794 11106 32-33 -2.4422160575011986 11106 34-35 -2.04371173593492 11106 36-37 -0.6238748618305934 11106 38-39 -2.412800401434353 11106 40-41 -0.6254659305252659 11106 42-43 -1.5225810518912226 11106 44-45 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11108 5 0.2513942206786517 11108 6 1.1364302299937066 11108 7 1.366413530647101 11108 8 0.3771707430426332 11108 9 0.9562564657009958 11108 10-11 0.1248773689263345 11108 12-13 0.7325204385470485 11108 14-15 0.48805047361305753 11108 16-17 0.4871634540223795 11108 18-19 0.011222706205131772 11108 20-21 0.26761919296366443 11108 22-23 -0.4272396509932719 11108 24-25 0.6385994030574977 11108 26-27 -0.35838625911335953 11108 28-29 -0.5049026915276471 11108 30-31 -0.9579560541705803 11108 32-33 0.10731625542397438 11108 34-35 0.0789498286909307 11108 36-37 0.7336931653802878 11108 38-39 0.38928293189898255 11108 40-41 0.44426196063120216 11108 42-43 0.5448084038910821 11108 44-45 0.18855009568721215 11108 46-47 -0.29276563630756414 11108 48-49 0.23917213239285218 11108 50-51 0.36672741524837704 11108 52-53 1.0990259083449985 11108 54-55 0.7103911979330491 11108 56-57 0.8625620560558218 11108 58-59 0.09710373075163403 11108 60-61 0.207911898638379 11108 62-63 -0.5038221723575447 11108 64-65 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0.581175327598153 11109 46-47 0.6297761076627495 11109 48-49 -0.23740486204128786 11109 50-51 0.2308276007771397 11109 52-53 1.3629405651168014 11109 54-55 -0.33970156644542726 11109 56-57 1.0121446163526713 11109 58-59 0.8480313745364185 11109 60-61 0.6916781324046113 11109 62-63 0.03444313376490271 11109 64-65 0.7841554785774107 11109 66-67 -0.33975895343891693 11109 68-69 0.45481617388774964 11109 70-71 1.258736296459361 11109 72-73 -0.47442996954777783 11109 74-75 0.6544591050681419 11109 76 1.8495298500851973 11110 1 0.0832856694589772 11110 2 1.4575919351490008 11110 3 0.9608210091697629 11110 4 0.9797650798256008 11110 5 1.219390510103512 11110 6 -0.770805391527631 11110 7 0.6957270742463564 11110 8 1.7885807615955045 11110 9 0.582879842324374 11110 10-11 0.4122150312639974 11110 12-13 1.4514443717567005 11110 14-15 -0.6875061126578146 11110 16-17 -0.8644135404117606 11110 18-19 0.30273476557433554 11110 20-21 0.2356154823885248 11110 22-23 0.2951629464093273 11110 24-25 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0.47506456948650566 21610 66-67 -0.41884986252982515 21610 68-69 -0.07609291702134158 21610 70-71 0.4919181146411802 21610 72-73 -0.4030663331841353 21610 74-75 0.4299136505226926 21610 76 -0.15865196809662052 21611 1 0.10137472326231745 21611 2 -1.2566937791367145 21611 3 -0.942704037212426 21611 4 0.44034021897216746 21611 5 -1.0955445548315517 21611 6 -0.9656105863328222 21611 7 -0.6948109591488176 21611 8 0.47921155936916193 21611 9 -0.2478251669520688 21611 10-11 0.0534487974977651 21611 12-13 -0.5225816022692769 21611 14-15 -0.2364393223053085 21611 16-17 0.8034899846346235 21611 18-19 0.4908145429398303 21611 20-21 0.34925184602488457 21611 22-23 0.5625562673740774 21611 24-25 0.14880348469014848 21611 26-27 0.7844708837437793 21611 28-29 -0.05592309969090792 21611 30-31 0.3991868029722738 21611 32-33 0.11752033705662512 21611 34-35 0.038133502160313526 21611 36-37 1.1826727572170341 21611 38-39 -0.2943905374887734 21611 40-41 0.6483435932842667 21611 42-43 0.59582881205435 21611 44-45 -0.5869601083944147 21611 46-47 0.9929486494067135 21611 48-49 -0.0057258267908224525 21611 50-51 0.4687682315749129 21611 52-53 0.38474019405928317 21611 54-55 1.0063095652799916 21611 56-57 1.3319498111578696 21611 58-59 1.137920057282244 21611 60-61 1.758932306801647 21611 62-63 -0.2691282948065279 21611 64-65 -0.41064971622778046 21611 66-67 0.4756399333885426 21611 68-69 0.6343152462439647 21611 70-71 0.25293852280444895 21611 72-73 0.46040305457096764 21611 74-75 0.8529748750124782 21611 76 2.2609398686380686 21612 1 -0.5925028277580928 21612 2 -0.8893468403612026 21612 3 -0.26923464945732434 21612 4 -0.15149651572171052 21612 5 -1.177177207892779 21612 6 0.054797576932486436 21612 7 -0.12338238772024823 21612 8 0.7853340083487552 21612 9 -0.2274170036867602 21612 10-11 -1.803694059645096 21612 12-13 -0.6552346634937649 21612 14-15 0.7839688409600001 21612 16-17 -0.6863059337327257 21612 18-19 0.6234676041643183 21612 20-21 -0.24258488866899341 21612 22-23 -0.2639743448708245 21612 24-25 0.4447218520370839 21612 26-27 0.5701851694580711 21612 28-29 0.12775036969684805 21612 30-31 -1.1314254419256784 21612 32-33 -0.3926837445760256 21612 34-35 -1.2475807835539712 21612 36-37 -0.42957214074215244 21612 38-39 0.29744619720510457 21612 40-41 -0.6883911005932859 21612 42-43 -0.8429466981497313 21612 44-45 0.6477337691566021 21612 46-47 0.2174384453250866 21612 48-49 1.29019254055612 21612 50-51 0.09121721116675019 21612 52-53 0.6194340716103 21612 54-55 -0.5753230877812285 21612 56-57 -0.5762134541482595 21612 58-59 0.8522057715679594 21612 60-61 0.707911898638379 21612 62-63 -0.5548425805208126 21612 64-65 -0.2779966550032924 21612 66-67 0.01645625991915267 21612 68-69 -0.7024194476335879 21612 70-71 0.30395893096770976 21612 72-73 -0.4783724556331137 21612 74-75 -0.014372063763019582 21612 76 0.09767456251562123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 11.0 12 28.0 13 35.0 14 59.0 15 102.0 16 210.0 17 266.0 18 326.0 19 362.0 20 419.0 21 444.0 22 583.0 23 637.0 24 833.0 25 959.0 26 1062.0 27 1236.0 28 1401.0 29 1644.0 30 1931.0 31 2348.0 32 3169.0 33 4317.0 34 7127.0 35 13973.0 36 27390.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 72.21548099444084 8.764850297711432 4.898834551457516 14.120834156390213 2 62.00722408781781 10.82061122555521 16.210457995880013 10.96170669074696 3 61.509157095690945 11.14654175014815 15.521912125744278 11.822389028416627 4 59.74856078564172 10.504853820973022 16.43667456823569 13.309910825149565 5 58.89465812568784 15.770240144481756 18.072918136411094 7.262183593419308 6 65.47437634044475 18.283666328027994 9.27728863302856 6.964668698498702 7 64.33106639952591 14.156108022688151 10.333831870643676 11.178993707142252 8 64.81361289048169 17.1050032451957 9.127465643254226 8.953918221068374 9 62.90035838248159 9.459039986454835 9.19236955724243 18.448232073821146 10-11 64.39879222281796 12.506702034596609 12.94409797669103 10.150407765894403 12-13 63.604424753788415 11.543020007336963 12.44955837119395 12.402996867680672 14-15 64.74094872590796 11.526794028839914 10.345119507859017 13.38713773739312 16-17 64.35646358326044 12.24144256003612 11.138781499562604 12.263312357140842 18-19 62.91420629713503 12.00660331435586 12.526543771649488 12.552646616859617 20-21 64.09493036840546 11.823966813878346 12.239498821836243 11.841603995879954 22-23 64.60549707932387 11.799108276659988 11.214267573440189 12.381127070575952 24-25 63.373475276372695 13.535383464905784 11.188949325206178 11.902191933515347 26-27 64.13751261049616 13.233437039231871 11.739931003830769 10.889119346441195 28-29 63.55080847701555 12.226627536190987 12.772666986483056 11.449897000310411 30-31 63.4807085864251 12.123713376649947 12.005192349749906 12.390385687175037 32-33 63.54112285355495 12.122387933345562 13.088200019753643 11.248289193345844 34-35 63.7807940852781 13.002652594745607 11.911279171487427 11.305274148488866 36-37 64.20690239015718 12.063662273894517 12.75855743996388 10.970877895984422 38-39 64.3971300979915 11.806246340310553 13.22567673389913 10.570946827798824 40-41 64.33882665011147 12.04531986341959 12.709174027146766 10.906679459322177 42-43 63.21191982899109 12.099021496045772 13.512102548907562 11.176956126055577 44-45 62.28447667691961 11.740553658605412 13.99878657899935 11.976183085475633 46-47 63.41650969685425 11.354737666405638 13.530445088784948 11.69830754795516 48-49 63.411123966475714 11.358184947935774 14.338826650111466 10.891864435477045 50-51 64.15963540931796 11.411801224708638 13.153624742500778 11.274938623472641 52-53 62.42883447268726 11.295477152955618 14.115995400255384 12.159692974101745 54-55 62.10246352682225 11.777943956881225 13.42664446764681 12.692948048649717 56-57 62.668961819567116 12.081299207043486 12.606879814882749 12.642859158506644 58-59 61.891256957819216 12.822846338899588 13.631329058110579 11.654567645170621 60-61 61.73279340801987 12.04602534074555 13.623472641589299 12.597708609645286 62-63 61.854135508084774 13.007590936027317 13.25874086406863 11.879532691819286 64-65 62.090908449561546 12.712085617332288 12.844716290291858 12.35228964281431 66-67 62.22424460482409 13.837329890579694 11.60306743705334 12.335358067542876 68-69 62.69012613934588 13.540931794452128 11.171233456556706 12.597708609645286 70-71 62.855913310946185 13.471795016508171 10.756412788892964 12.91587888365268 72-73 62.5786607218444 12.776194373112848 11.464712024155544 13.180432880887208 74-75 62.87417545592437 12.415958234858373 11.61522452291086 13.094641786306397 76 64.68098315320145 10.814967406947542 11.792758980726358 12.711290459124644 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 1.0 6 0.5 7 1.0 8 2.0 9 1.0 10 0.0 11 1.0 12 2.0 13 2.5 14 5.0 15 8.0 16 6.5 17 4.0 18 13.0 19 22.5 20 26.5 21 30.0 22 26.0 23 36.0 24 69.5 25 89.0 26 123.5 27 138.5 28 171.0 29 223.0 30 234.0 31 295.0 32 398.5 33 452.0 34 535.5 35 729.5 36 884.5 37 929.0 38 1060.5 39 1351.5 40 1509.5 41 1526.0 42 1544.0 43 1802.0 44 2419.5 45 2454.5 46 2130.0 47 2081.0 48 1897.0 49 1679.5 50 1597.0 51 1569.5 52 1395.0 53 1241.0 54 1234.0 55 1161.0 56 975.5 57 856.0 58 849.0 59 764.0 60 645.5 61 550.5 62 489.0 63 424.5 64 341.5 65 327.5 66 304.0 67 276.0 68 237.5 69 171.5 70 143.0 71 142.0 72 128.0 73 112.5 74 96.0 75 81.0 76 75.5 77 54.0 78 40.5 79 43.0 80 45.5 81 52.0 82 51.5 83 47.0 84 51.0 85 61.5 86 70.0 87 72.0 88 74.5 89 74.0 90 81.5 91 103.5 92 115.0 93 146.0 94 184.0 95 241.5 96 292.0 97 370.0 98 723.5 99 17959.0 100 34919.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.002821909303834975 5 0.0 6 0.002821909303834975 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.054773259587438E-4 20-21 0.0014109546519174875 22-23 0.0 24-25 7.054773259587438E-4 26-27 7.054773259587438E-4 28-29 0.0 30-31 7.054773259587438E-4 32-33 0.0014109546519174875 34-35 0.0 36-37 0.0 38-39 7.054773259587438E-4 40-41 0.0 42-43 7.054773259587438E-4 44-45 0.0 46-47 7.054773259587438E-4 48-49 0.0 50-51 0.0 52-53 7.054773259587438E-4 54-55 0.0 56-57 0.0 58-59 7.054773259587438E-4 60-61 0.0 62-63 0.0 64-65 7.054773259587438E-4 66-67 7.054773259587438E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.002116431977876231 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70874.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.974207748962947 #Duplication Level Percentage of deduplicated Percentage of total 1 68.29330936175677 15.006913677794396 2 6.3439065108514185 2.788046392188955 3 5.091819699499165 3.3566611169117024 4 4.944137665339668 4.345740327905861 5 4.494670604854244 4.938341281711206 6 3.4223706176961604 4.5122329768321245 7 2.536278412739181 3.9012896125518526 8 1.7850263259278285 3.137963145864492 9 1.123667651213561 2.2222535767700426 >10 1.9262874020803904 5.629709061150775 >50 0.019262874020803903 0.3809577560177216 >100 0.006420958006934635 0.1467392837994187 >500 0.006420958006934635 1.2077771820413692 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.006420958006934635 48.42537460846009 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 34321 48.42537460846009 No Hit CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG 856 1.2077771820413692 No Hit ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC 104 0.1467392837994187 No Hit CTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGG 98 0.13827355588791376 No Hit TCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGT 97 0.13686260123599628 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 75 0.10582159889381156 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.02680813838643226 0.0 5 0.0 0.0 0.0 0.052205322120947036 0.0 6 0.0 0.0 0.0 0.052205322120947036 0.0 7 0.0 0.0 0.0 0.06631486864012191 0.0 8 0.0 0.0 0.0 0.06631486864012191 0.0 9 0.0 0.0 0.0 0.07478059655162683 0.0 10 0.0 0.0 0.0 0.08183536981121427 0.0 11 0.0 0.0 0.0 0.08747918841888422 0.0 12 0.0 0.0 0.0 0.08747918841888422 0.0 13 0.0 0.0 0.0 0.08747918841888422 0.0 14 0.0 0.0 0.0 0.09594491633038914 0.0 15 0.0 0.0 0.0 0.09594491633038914 0.0 16 0.0 0.0 0.0 0.09594491633038914 0.0 17 0.0 0.0 0.0 0.12557496402065638 0.0 18 0.0 0.0 0.0 0.12839687332449135 0.0 19 0.0 0.0 0.0 0.16225978497051105 0.0 20 0.0 0.0 0.0 0.17495837683776844 0.0 21 0.0 0.0 0.0 0.17495837683776844 0.0 22 0.0 0.0 0.0 0.22151988035104553 0.0 23 0.0 0.0 0.0 0.24268420012980782 0.0 24 0.0 0.0 0.0 0.332985297852527 0.0 25 0.0 0.0 0.0 0.3583824815870418 0.0 26 0.0 0.0 0.0 0.36684820949854674 0.0 27 0.0 0.0 0.0 0.39788921184073145 0.0 28 0.0 0.0 0.0 0.4472726246578435 0.0 29 0.0 0.0 0.0 0.4867793549115331 0.0 30 0.0 0.0 0.0 0.5234641758613878 0.0 31 0.0 0.0 0.0 0.55591613285549 0.0 32 0.0 0.0 0.0 0.619409092191777 0.0 33 0.0 0.0 0.0 0.729463555041341 0.0 34 0.0 0.0 0.0 0.8268194260236476 0.0 35 0.0 0.0 0.0 0.958038208651974 0.0 36 0.0 0.0 0.0 1.189434771566442 0.0 37 0.0 0.0 0.0 1.4885571577729493 0.0 38 0.0 0.0 0.0 2.0458842452803565 0.0 39 0.0 0.0 0.0 2.567937466489827 0.0 40 0.0 0.0 0.0 3.07729209583204 0.0 41 0.0 0.0 0.0 3.520331856534131 0.0 42 0.0 0.0 0.0 4.076247989389621 0.0 43 0.0 0.0 0.0 4.668848943194965 0.0 44 0.0 0.0 0.0 5.286847080734825 0.0 45 0.0 0.0 0.0 5.892146626407428 0.0 46 0.0 0.0 0.0 6.41278889296498 0.0 47 0.0 0.0 0.0 7.109800491012219 0.0 48 0.0 0.0 0.0 7.7334424471597485 0.0 49 0.0 0.0 0.0 8.374015859130287 0.0 50 0.0 0.0 0.0 9.075260321133278 0.0 51 0.0 0.0 0.0 9.786381465699693 0.0 52 0.0 0.0 0.0 10.316900414820667 0.0 53 0.0 0.0 0.0 10.713378672009481 0.0 54 0.0 0.0 0.0 11.147952704800067 0.0 55 0.0 0.0 0.0 11.514800914298615 0.0 56 0.0 0.0 0.0 11.962073538956458 0.0 57 0.0 0.0 0.0 12.310579337980077 0.0 58 0.0 0.0 0.0 12.749386234726416 0.0 59 0.0 0.0 0.0 13.093659169794282 0.0 60 0.0 0.0 0.0 13.43228828625448 0.0 61 0.0 0.0 0.0 13.773739312018511 0.0 62 0.0 0.0 0.0 14.08979315404803 0.0 63 0.0 0.0 0.0 14.421367497248639 0.0 64 0.0 0.0 0.0 14.751530885797331 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAATT 15 0.0022113086 70.03954 18 CCCAAAG 15 0.002217515 69.99012 51 GCTAGTG 15 0.002217515 69.99012 65 CTTGTGC 25 2.37736E-4 55.992096 1 AGGGCAT 20 0.006909807 52.52966 29 CCTGACT 20 0.006909807 52.52966 31 AAGTACA 20 0.0069291447 52.492588 9 CGAAAGC 20 0.0069291447 52.492588 50 ACTCTTT 20 0.0069291447 52.492588 11 ACACTCT 20 0.0069291447 52.492588 9 GGTCTCT 20 0.0069291447 52.492588 58 GCTAACA 20 0.0069291447 52.492588 24 TGTGCGC 30 5.8459886E-4 46.66008 3 GTCGCCG 200 0.0 45.493576 51 TCTCGGT 185 0.0 45.398994 43 TACGTGT 210 0.0 45.025425 33 ACGTGTA 210 0.0 44.993652 34 ATCTCGG 195 0.0 44.86546 42 CTCGGTG 195 0.0 44.86546 44 TCGGTGG 195 0.0 44.86546 45 >>END_MODULE