SRR1780029_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780029_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	70874
Sequences flagged as poor quality	0
Sequence length	76
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT	21634	30.524592939582924	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG	4001	5.645229562321867	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC	2415	3.407455484380732	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	1653	2.3323080396196065	No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT	1044	1.473036656601857	TruSeq Adapter, Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC	665	0.9382848435251291	No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT	452	0.6377515026667043	No Hit
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC	269	0.3795468013658041	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGACGT	202	0.28501283968733243	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATATCGTATGCCGT	188	0.26525947456048765	No Hit
TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT	182	0.2567937466489827	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC	167	0.23562942687022037	No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC	162	0.22857465361063295	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG	158	0.222930835002963	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCAGT	130	0.18342410474927337	No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG	125	0.17636933148968592	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT	118	0.1664926489262635	No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG	98	0.13827355588791376	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTCGGATCTCGTATGCCGT	98	0.13827355588791376	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC	96	0.1354516465840788	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGA	90	0.12698591867257386	No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT	82	0.11569828145723397	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCAG	76	0.10723255354572903	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGC	15	0.002214938	70.01059	51
GTCTTAA	15	0.002214938	70.01059	49
CGGAACT	15	0.0022211545	69.96119	34
ATGTCAC	15	0.0022211545	69.96119	40
TATCTCG	15	0.0022211545	69.96119	36
ATGAATT	15	0.0022211545	69.96119	41
GAATTAC	15	0.0022211545	69.96119	43
TCGAATG	50	1.4551915E-11	62.96507	40
AGGGGGG	2615	0.0	62.51424	70
TCTTTAT	90	0.0	62.187717	1
TATGACG	40	1.0526492E-8	61.216038	43
TTCTGCA	35	2.932902E-7	60.00908	52
CGACTTC	30	8.292052E-6	58.34216	48
CAGCTAC	30	8.326881E-6	58.30099	10
GAACTCG	25	2.3822216E-4	55.968952	36
CCATAAT	25	2.3822216E-4	55.968952	46
CCGACTT	25	2.3822216E-4	55.968952	47
CGTAAGC	25	2.3822216E-4	55.968952	41
AGGATCT	25	2.3822216E-4	55.968952	34
AGTCTTC	45	2.6522684E-8	54.452682	48

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content