FastQCFastQC Report
Wed 25 May 2016
SRR1780028_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780028_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197894
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4273021.592367631156073No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG11350.5735393695614824No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTG150.002222398369.9982358
CGTTAAT150.002222398369.9982336
GCTCTAT150.002222398369.998231
TGCGTAT150.002222398369.9982356
TGCCGTT252.3835033E-456.0127419
ATGTGTC252.3864831E-455.9985859
CCTGACG200.00693733152.51194414
CGGATCC200.00694427152.49867268
TCTCGGC200.00694427152.49867222
TATAGAC200.00694427152.4986723
ACGTACA200.00694427152.49867211
TGGGACA200.00694427152.49867227
TGCAACA200.00694427152.4986722
TCGGTGG2400.048.12378745
GCCGTAT2150.047.2081154
TACTTAC305.860966E-446.67728419
TCGCCGT2100.046.6654952
CGTATCA2250.046.66548556
TGGTCGC2250.046.66548549
GTCGCCG2200.046.13519751
Produced by FastQC (version 0.11.5)