FastQCFastQC Report
Wed 25 May 2016
SRR1780028_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780028_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197894
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT2643613.358666760993259No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG43852.2158327185260793No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC31661.599846382406743No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21561.0894721416515913No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT12750.6442843138245727TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC8550.4320494810353018No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT5960.3011713341485846No Hit
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC3850.19454859672349845No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT2740.13845796234347682No Hit
TATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCT2370.11976108421680293No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAT150.002223607969.9886251
GTGCTCG150.002223607969.9886254
TGTCGTC150.002223607969.98862516
AGGGGGG36100.061.18280870
ATATCCG352.9666262E-759.99025310
TATCCGA352.9666262E-759.99025311
GTGTCTC308.362633E-658.32385638
TTGGACG252.38512E-456.0050649
ATTCCGT252.3881017E-455.99090644
ACGGCGT252.3881017E-455.9909068
TCGTATT252.3881017E-455.99090640
AATATCG700.054.99106636
GTGCAAC200.00693415652.51801729
CGACATG200.00693415652.51801729
TCGAGAA200.00693415652.51801720
TCGACAT200.00693415652.51801728
TAACGAG200.006941094552.5047464
CTACGCG200.006941094552.5047465
GTCCGTA200.006941094552.5047459
ACGGTAA200.006941094552.5047457
Produced by FastQC (version 0.11.5)