FastQCFastQC Report
Wed 25 May 2016
SRR1780027_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780027_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180165
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3130217.374073765714762No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG6490.36022534898565206No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGT150.002221851670.000017
TTGTCGA150.002221851670.0000131
CGAGAAC150.002221851670.000013
GTTCTAG308.351559E-658.3333361
TCGCTAC252.3856098E-456.00000413
CCCGAAC252.3856098E-456.0000048
TCCATGG200.006942572552.516
GTGGGAC200.006942572552.59
TTGTATC200.006942572552.561
TTCCCTT200.006942572552.533
TCCGTAC200.006942572552.540
CGCCGTG200.006942572552.543
TAGTGAC200.006942572552.567
GCCGTGT200.006942572552.544
GCATCCG200.006942572552.537
TGGTATC200.006942572552.58
ATTTCCC352.0804062E-550.049
AGATCTC1200.043.75000440
CGGTGGT1200.043.75000446
TCTCGGT1300.043.07692743
Produced by FastQC (version 0.11.5)