FastQCFastQC Report
Wed 25 May 2016
SRR1780027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180165
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT177319.841534149252075Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG32511.8044570255043988No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC18311.0162906224849444No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12880.7149002303444065No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT10740.5961202231287985Illumina PCR Primer Index 6 (95% over 24bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC8040.4462575972025643No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT5070.2814087086837066Illumina PCR Primer Index 6 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC3070.17039935614575527Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGACGT2140.11878000721560793No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCG150.002221851670.0000136
CTTAAGA207.910511E-570.010
GCCGACT453.8198777E-1062.22222546
CCGACTT401.0604708E-861.2547
AGGGGGG22550.058.6696270
ACGTATG308.351559E-658.33333640
CACGTAT308.351559E-658.33333639
AGAACGG252.3856098E-456.00000425
CTTTATA1000.056.0000042
CGACTTC509.749783E-1056.00000448
GCCGTAT252.3856098E-456.00000446
CGAACTG200.006942572552.526
GTCTCGC200.006942572552.529
CGTCTTA407.4488526E-752.548
GTATGAC800.052.542
TACGACA200.006942572552.549
GAAACGC200.006942572552.551
TCGTCTT200.006942572552.547
AATCTCG407.4488526E-752.536
GGGTTGC200.006942572552.551
Produced by FastQC (version 0.11.5)