FastQCFastQC Report
Wed 25 May 2016
SRR1780026_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780026_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344826
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4389412.729318554865351No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG9960.28884132867011186No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.0022235870.01
GTGTCGC150.0022235870.019
GGTCTAA207.9207595E-570.01
AGTGTCG150.0022235870.018
TCGTTCC252.3886823E-456.00000435
ACTAGTG252.3886823E-456.0000042
ACCGACG200.00694793152.569
TCAGCGC200.00694793152.53
TACCGAC200.00694793152.568
TGCCGGC407.468934E-752.519
GTCGCCG2000.049.00000451
TGGTCGC2000.047.25000449
TCGCCGT1950.046.66666852
GCCGTAT1950.046.66666854
CTCGTTC305.873647E-446.66666434
ATGCAGT305.873647E-446.66666446
CTCGGTG2150.045.58139444
CGGTGGT2400.043.7546
TCGGTGG2350.043.1914945
TAGATCT2400.042.29166439
Produced by FastQC (version 0.11.5)