FastQCFastQC Report
Wed 25 May 2016
SRR1780026_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780026_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344826
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT270367.840476066189904No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG44421.2881859256552581No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC33300.9657044422404343No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20460.5933427293765552No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT13360.3874417822321983RNA PCR Primer, Index 37 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC8640.25056115258130185No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT5950.17255079373365118No Hit
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC4010.1162905349364607No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACT150.002222575270.0079734
CGGTTTA150.002223854269.99782662
GACGAAT150.002223854269.99782646
AGGGGGG30850.059.3336570
GCAGCGT252.3890492E-455.9982641
CGACGCT200.006944852.5059826
AAGCGAC200.006944852.5059831
AGCGACA200.006944852.5059832
CAGGTAT200.006948784552.498371
ACATGTC200.006948784552.4983764
GTCTTAA200.006948784552.4983749
GCCGTAA200.006948784552.4983746
CGTCTAC352.0847918E-549.99844748
ACGTATG352.0847918E-549.99844740
CGAATGC800.048.12350541
ATGACGA305.8745476E-446.6652244
TATACCG451.6826962E-646.6652156
TGCCGTA451.6826962E-646.66521545
GCCGTCT43000.046.39390646
CCGTCTT42850.046.31127547