##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780026_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 344826 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.471304368000094 32.0 27.0 32.0 13.0 32.0 2 29.377306235608685 32.0 27.0 32.0 22.0 32.0 3 30.4537070870526 32.0 32.0 32.0 27.0 32.0 4 30.71787510222547 32.0 32.0 32.0 27.0 32.0 5 30.600006960032015 32.0 32.0 32.0 27.0 32.0 6 33.64897368527895 37.0 32.0 37.0 22.0 37.0 7 34.47464808338118 37.0 37.0 37.0 27.0 37.0 8 33.715694292193746 37.0 32.0 37.0 22.0 37.0 9 34.62623468067953 37.0 37.0 37.0 27.0 37.0 10-11 34.61088201005725 37.0 37.0 37.0 29.5 37.0 12-13 34.527808227917845 37.0 37.0 37.0 27.0 37.0 14-15 34.42204474140581 37.0 37.0 37.0 27.0 37.0 16-17 33.948378312540235 37.0 34.5 37.0 24.5 37.0 18-19 34.220285013311056 37.0 37.0 37.0 27.0 37.0 20-21 34.34995475979189 37.0 37.0 37.0 27.0 37.0 22-23 34.23683248942945 37.0 37.0 37.0 27.0 37.0 24-25 34.294761705903845 37.0 37.0 37.0 27.0 37.0 26-27 34.538175775608565 37.0 37.0 37.0 29.5 37.0 28-29 34.494081072772936 37.0 37.0 37.0 27.0 37.0 30-31 34.39274155661116 37.0 37.0 37.0 27.0 37.0 32-33 34.248277392076005 37.0 37.0 37.0 27.0 37.0 34-35 34.226334441138434 37.0 37.0 37.0 27.0 37.0 36-37 34.133618114643326 37.0 37.0 37.0 27.0 37.0 38-39 33.84576569052217 37.0 32.0 37.0 27.0 37.0 40-41 33.73957735205582 37.0 32.0 37.0 24.5 37.0 42-43 33.86571488228846 37.0 37.0 37.0 27.0 37.0 44-45 33.9654593911132 37.0 34.5 37.0 27.0 37.0 46-47 33.50253171164587 37.0 32.0 37.0 22.0 37.0 48-49 33.22269637440333 37.0 32.0 37.0 22.0 37.0 50-51 33.18203963738233 37.0 32.0 37.0 22.0 37.0 52-53 32.93821376578332 37.0 32.0 37.0 17.5 37.0 54-55 32.81187903464356 37.0 32.0 37.0 22.0 37.0 56-57 32.643640560746576 37.0 32.0 37.0 17.5 37.0 58-59 32.797435518203386 37.0 32.0 37.0 17.5 37.0 60-61 32.87655513215361 37.0 32.0 37.0 17.5 37.0 62-63 33.15079199364317 37.0 32.0 37.0 22.0 37.0 64-65 33.12362321866681 37.0 32.0 37.0 22.0 37.0 66-67 33.030444340043964 37.0 32.0 37.0 22.0 37.0 68-69 32.721150667293074 37.0 32.0 37.0 22.0 37.0 70-71 31.342968627655686 37.0 29.5 37.0 13.0 37.0 72-73 31.881318404064658 37.0 32.0 37.0 13.0 37.0 74-75 32.367775341766574 37.0 32.0 37.0 13.0 37.0 76 31.844231583465284 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.5347513461906779 11101 2 0.15608064463411964 11101 3 0.4486115917365332 11101 4 0.05128335184733501 11101 5 -0.5604675179178322 11101 6 0.17549641232110957 11101 7 -0.04304115002906883 11101 8 -0.35298807137997557 11101 9 -0.17421560967719785 11101 10-11 0.30636134008218363 11101 12-13 0.2193084537262706 11101 14-15 0.41144632947894877 11101 16-17 0.01005803509648473 11101 18-19 0.0013415316095688468 11101 20-21 0.23428887190166137 11101 22-23 -0.11861575722270601 11101 24-25 0.29710273578000823 11101 26-27 0.036652457605541144 11101 28-29 0.3918485977893411 11101 30-31 0.30038932987471867 11101 32-33 0.01238539012008033 11101 34-35 0.057350127832663134 11101 36-37 0.0604260418515139 11101 38-39 0.18292555668453048 11101 40-41 -0.12512455476272066 11101 42-43 0.2939738978809956 11101 44-45 -0.046376167700408644 11101 46-47 -0.16824110663850433 11101 48-49 0.29174140077710575 11101 50-51 0.003095394979148125 11101 52-53 -0.669752629847828 11101 54-55 -0.76541009734742 11101 56-57 -0.2660384304448513 11101 58-59 -0.1715325467644675 11101 60-61 -0.357149533581655 11101 62-63 -0.7192355855872492 11101 64-65 -0.18503051816765037 11101 66-67 -0.6172505043232448 11101 68-69 0.1476214860144438 11101 70-71 -0.3625784602304556 11101 72-73 -0.0809534569970971 11101 74-75 0.3365613743899729 11101 76 -0.9918392573933232 11102 1 -0.23390037068216785 11102 2 -0.1975723234024116 11102 3 0.06911802594159866 11102 4 -0.1415144895806435 11102 5 0.3117616228044575 11102 6 -0.18604364579428534 11102 7 -0.10956252819428158 11102 8 -0.9631375109814684 11102 9 -0.1402803668378425 11102 10-11 0.3653380939219488 11102 12-13 -0.5877691718445064 11102 14-15 -0.15787704121428447 11102 16-17 -0.026668822520782953 11102 18-19 -0.7031312786851629 11102 20-21 -0.39099132735113784 11102 22-23 -0.14533846373992532 11102 24-25 -0.2299001699900174 11102 26-27 0.061932656858346036 11102 28-29 -0.3551418546805607 11102 30-31 -0.7445255725744317 11102 32-33 -0.3222347842261186 11102 34-35 -0.8340778505816218 11102 36-37 -0.772056315973316 11102 38-39 -0.24170084431173677 11102 40-41 -0.7502594655140626 11102 42-43 -0.21451510170389554 11102 44-45 -0.05838322457048406 11102 46-47 -0.6872531448286239 11102 48-49 -0.29277499606806145 11102 50-51 -0.2221848042736525 11102 52-53 -0.670873426859032 11102 54-55 -0.2082286942756042 11102 56-57 -0.8624477289089469 11102 58-59 -0.07292316527836817 11102 60-61 -0.7703500317136687 11102 62-63 -0.6302878894477786 11102 64-65 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11105 62-63 0.41282610272443065 11105 64-65 0.17548084546871223 11105 66-67 -0.12833004977778728 11105 68-69 -0.7157713711284153 11105 70-71 -0.6121320316590264 11105 72-73 -1.1000687167373577 11105 74-75 -1.0259792100256107 11105 76 -0.643137958692023 11106 1 -0.5601284354691138 11106 2 -1.106923839670813 11106 3 -0.6530598310111237 11106 4 -0.3448234561428798 11106 5 -0.3051067352027914 11106 6 -0.2750335509891215 11106 7 -0.7708234574077863 11106 8 -1.2818303053791595 11106 9 -0.2338364820783383 11106 10-11 -0.5916105310959665 11106 12-13 -0.4903328018791129 11106 14-15 -0.28790140798538033 11106 16-17 -0.4938453485154142 11106 18-19 -0.5666466461319715 11106 20-21 -0.6253116172952389 11106 22-23 -0.3044596218782232 11106 24-25 -0.48208601269125495 11106 26-27 -0.6095766496101049 11106 28-29 -0.8087221319293647 11106 30-31 -0.4282694552863049 11106 32-33 -0.8335991186446918 11106 34-35 -0.31633515549388136 11106 36-37 -0.36360575594710554 11106 38-39 -0.5436235668376881 11106 40-41 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21610 18-19 0.05249192442663286 21610 20-21 0.17065250826529876 21610 22-23 0.7562907149059939 21610 24-25 0.4041547372764569 21610 26-27 0.535268245110224 21610 28-29 0.5136218862292949 21610 30-31 0.5873702501889753 21610 32-33 0.6645739423086283 21610 34-35 0.6442749314241354 21610 36-37 0.2403175495207961 21610 38-39 0.36335017322025465 21610 40-41 0.4152233689185323 21610 42-43 0.08104834606655942 21610 44-45 0.6181487107133492 21610 46-47 0.18469566852049724 21610 48-49 0.654741774888592 21610 50-51 0.7448088255814724 21610 52-53 0.8617101460594014 21610 54-55 0.9135087204602854 21610 56-57 0.36405883854130394 21610 58-59 1.2206672287686402 21610 60-61 0.497957088191626 21610 62-63 -0.3798509989804444 21610 64-65 0.11934658620945271 21610 66-67 0.2283443329382635 21610 68-69 0.7502963831337865 21610 70-71 0.6706519924444372 21610 72-73 0.20558788232202474 21610 74-75 0.7415931738662138 21610 76 0.43769684436500356 21611 1 0.7468850174456847 21611 2 0.4780806446341188 21611 3 0.13370250082744306 21611 4 0.14201062457460623 21611 5 0.26535066390034956 21611 6 0.21676913959383626 21611 7 1.161140668152754 21611 8 0.5617392013473008 21611 9 0.36996620850462136 21611 10-11 0.47245224917308803 21611 12-13 0.42330845372626413 21611 14-15 0.1432645112971258 21611 16-17 0.5512398532783038 21611 18-19 -0.1271130138449763 21611 20-21 0.20665250826530013 21611 22-23 -0.32634302994997455 21611 24-25 0.36073909941637083 21611 26-27 0.38828882124190756 21611 28-29 0.30475768869843733 21611 30-31 -0.21724703376164456 21611 32-33 0.4902035719382596 21611 34-35 0.09207740055992986 21611 36-37 0.288062405487878 21611 38-39 0.27410737486634673 21611 40-41 1.1614208997827404 21611 42-43 -0.11893519302809352 21611 44-45 0.18516928684504563 21611 46-47 0.0013952569978599172 21611 48-49 -0.10716769013198757 21611 50-51 0.2674590313427814 21611 52-53 0.5577019156067209 21611 54-55 0.1898626299253081 21611 56-57 -0.3144020668084906 21611 58-59 -0.04098709221901231 21611 60-61 -0.028240442672569088 21611 62-63 -0.02278104013270621 21611 64-65 0.5706058454687124 21611 66-67 0.5262949502222085 21611 68-69 -0.05237851398555904 21611 70-71 0.4844215397695386 21611 72-73 0.5645919975483586 21611 74-75 0.41528864711724367 21611 76 0.6374334698794009 21612 1 0.8288850174456854 21612 2 0.36024731130078536 21612 3 -0.12146416583922459 21612 4 0.057343957907942666 21612 5 0.4296839972336812 21612 6 0.011102472927170481 21612 7 -0.09985933184724871 21612 8 0.4364058680139635 21612 9 0.018966208504622273 21612 10-11 0.07170224917309298 21612 12-13 0.1255584537262706 21612 14-15 0.27451451129713433 21612 16-17 0.36573985327830627 21612 18-19 -0.4566130138449722 21612 20-21 0.2810691749319645 21612 22-23 0.03782363671668776 21612 24-25 0.15865576608304366 21612 26-27 -0.43512784542475913 21612 28-29 -0.6734923113015654 21612 30-31 -0.2569970337616425 21612 32-33 -0.22454642806173553 21612 34-35 -0.06158926610673632 21612 36-37 0.07614573882121078 21612 38-39 0.721940708199682 21612 40-41 -0.08932910021727025 21612 42-43 0.3759814736385678 21612 44-45 -0.09808071315495681 21612 46-47 0.5870619236645283 21612 48-49 0.14458230986801368 21612 50-51 -0.1397076353238873 21612 52-53 0.45903524894005443 21612 54-55 0.3285292965919737 21612 56-57 -0.7357354001418273 21612 58-59 -0.0794870922190114 21612 60-61 0.2400095573274328 21612 62-63 -0.23419770679937102 21612 64-65 -0.12972748786462063 21612 66-67 -0.09603838311112156 21612 68-69 -0.4982118473188919 21612 70-71 0.11242153976954228 21612 72-73 0.14859199754835473 21612 74-75 0.22595531378390632 21612 76 0.48126680321273696 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 7.0 16 12.0 17 36.0 18 78.0 19 136.0 20 271.0 21 461.0 22 859.0 23 1339.0 24 2065.0 25 2999.0 26 4062.0 27 5702.0 28 7579.0 29 10428.0 30 14867.0 31 22016.0 32 34422.0 33 57209.0 34 88890.0 35 79505.0 36 11883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.48801679736439 19.769384251310846 12.177799174052248 28.564799777272516 2 21.937362423834646 20.798471037588925 32.95911649637915 24.305050042197273 3 17.88126524350456 23.02309327502255 34.83595741533964 24.259684066133246 4 12.426919239960094 26.02429065262279 36.120745655754824 25.428044451662295 5 12.012435257202183 34.64732937771514 39.57271203447536 13.76752333060732 6 25.233460552416968 43.43729336566013 18.79110935814299 12.538136723779914 7 25.30087638403137 29.051463636732727 21.253907767975733 24.39375221126017 8 22.78540481286214 41.26022979705707 17.765191719881912 18.189173670198883 9 22.18481205013543 18.18337364351876 18.397684629349296 41.23412967699651 10-11 22.31144674224167 28.77867775641416 28.200265063525343 20.709610437818824 12-13 22.71507948936565 21.735599983759926 28.991288359926454 26.55803216694797 14-15 20.723640328745514 21.81332034127357 22.23498228091849 35.22805704906243 16-17 25.894799116075934 29.39091019818691 21.962961029620736 22.751329656116418 18-19 26.18695814198982 25.689452918257473 23.415226182898056 24.708362756854655 20-21 20.45756410479488 24.5983481524015 22.87139020839496 32.072697534408654 22-23 21.072313597206698 29.695540775089935 22.979370665367455 26.252774962335913 24-25 25.848963031064116 29.82166628480611 23.159456269231995 21.169914414897782 26-27 25.923981459458517 30.968389420985154 23.70445355872815 19.403175560828174 28-29 21.259970415059314 30.309481683441135 23.902166661832528 24.528381239667024 30-31 25.077112938006564 25.363812098211504 29.316983118538577 20.24209184524336 32-33 31.335106398405983 24.68756253824803 24.17130476900365 19.806026294342338 34-35 22.595922392041995 34.14880658913604 23.943476117281982 19.311794901539976 36-37 20.218313004239818 30.68373614518627 23.687888964289236 25.41006188628468 38-39 19.730124367252422 25.382693565296073 29.52754364163903 25.359638425812474 40-41 19.567452347981177 23.1941534289878 30.822101546470233 26.416292676560786 42-43 25.030487141748896 22.34819868481153 30.762504803265493 21.85880937017408 44-45 19.556964962038826 27.827223005225825 31.749201046324814 20.86661098641054 46-47 23.8070725189124 22.391089676013266 27.771679473106474 26.03015833196786 48-49 23.01320086072396 21.676874713623683 27.12280976492492 28.187114660727442 50-51 18.852406721070917 22.344312783838806 32.11895274718264 26.684327747907638 52-53 16.726551942138933 22.904305359804656 37.608968001252805 22.760174696803606 54-55 16.033843989936706 22.994772597098464 33.21249646552162 27.758886947443212 56-57 21.554494150673094 23.029150934094297 27.217640201144928 28.198714714087686 58-59 16.40515000050752 23.09466946816486 35.91906396319191 24.581116568135712 60-61 21.702539831683225 27.789957833806035 27.53011663853654 22.9773856959742 62-63 16.733005337544206 35.29608797595278 23.91396708151899 24.05693960498403 64-65 16.967438353284077 36.8243150163497 22.05520471567469 24.15304191469153 66-67 17.137309606394208 36.52570096168347 21.25114555177894 25.085843880143383 68-69 17.045263408211678 36.585553293545146 21.30625300876384 25.06293028947933 70-71 21.889590692117185 31.979897107526696 21.21591759322093 24.914594607135193 72-73 31.105304522082598 23.873829458353512 20.680124935110882 24.340741084453 74-75 33.00701551882647 23.655697245125534 19.646497895924387 23.690789340123604 76 32.69694236519348 23.75083016974615 19.947565173762865 23.6046622912975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 0.5 12 0.0 13 0.0 14 14.0 15 18.0 16 19.0 17 30.0 18 32.5 19 51.5 20 73.5 21 79.0 22 125.0 23 200.0 24 368.5 25 508.0 26 714.5 27 1033.0 28 1330.5 29 1516.0 30 1863.5 31 2599.0 32 3617.0 33 4247.0 34 4824.5 35 6311.5 36 8082.0 37 8943.0 38 10535.0 39 13479.5 40 15874.0 41 17607.5 42 18299.0 43 19090.0 44 21045.0 45 23576.0 46 24943.0 47 30785.0 48 41124.0 49 33060.0 50 20499.0 51 19293.0 52 15847.5 53 12138.5 54 10669.0 55 9730.0 56 7540.0 57 5246.0 58 4203.0 59 3458.0 60 2352.0 61 1682.5 62 1374.0 63 1204.5 64 964.5 65 826.5 66 733.0 67 707.0 68 654.5 69 540.5 70 450.5 71 422.0 72 396.0 73 351.0 74 323.0 75 314.0 76 304.5 77 253.0 78 197.0 79 183.0 80 164.0 81 133.0 82 119.5 83 118.0 84 92.0 85 62.0 86 44.5 87 31.0 88 31.5 89 26.5 90 19.5 91 15.0 92 12.0 93 11.0 94 8.0 95 11.0 96 16.0 97 33.0 98 91.0 99 1044.5 100 1957.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0029000133400613644 2 0.004930022678104319 3 0.002030009338042955 4 5.800026680122729E-4 5 0.0 6 0.00406001867608591 7 0.0 8 0.0 9 0.0 10-11 8.700040020184093E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0015950073370337504 20-21 0.0 22-23 4.3500200100920464E-4 24-25 0.006670030682141138 26-27 0.019720090712417277 28-29 0.01624007470434364 30-31 0.011455052693242389 32-33 0.010730049358227047 34-35 0.004640021344098183 36-37 0.0 38-39 1.4500066700306823E-4 40-41 0.0024650113390521596 42-43 0.0024650113390521596 44-45 0.0 46-47 0.003625016675076705 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0024650113390521596 56-57 0.0 58-59 0.0030450140070644323 60-61 0.0 62-63 0.0013050060030276138 64-65 0.005365024679113524 66-67 0.00406001867608591 68-69 0.0 70-71 0.0 72-73 0.002030009338042955 74-75 0.005510025346116592 76 0.004930022678104319 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 344826.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.06481529815037 #Duplication Level Percentage of deduplicated Percentage of total 1 64.93000115700566 16.274584863090368 2 5.439083651509892 2.7265925423256947 3 3.967372440124957 2.9832437229211255 4 3.928034247367812 3.9382181158033323 5 3.6596089320837675 4.5863710973070475 6 3.3194492653014005 4.992082963581632 7 2.79301168575726 4.900442542035694 8 2.1971537660534537 4.405700266221225 9 1.7308804813143583 3.904577961058621 >10 7.72301284276293 33.8092255224374 >50 0.2591692699294227 4.280129688596568 >100 0.04512322110378341 1.7005678226119842 >500 0.0023140113386555593 0.423111946314953 >1k 0.0046280226773111185 3.2346748795044453 >5k 0.0 0.0 >10k+ 0.0011570056693277796 7.840476066189904 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT 27036 7.840476066189904 No Hit TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG 4442 1.2881859256552581 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC 3330 0.9657044422404343 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 2046 0.5933427293765552 No Hit ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT 1336 0.3874417822321983 RNA PCR Primer, Index 37 (95% over 22bp) TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC 864 0.25056115258130185 No Hit ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT 595 0.17255079373365118 No Hit CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC 401 0.1162905349364607 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.900013340061364E-4 0.0 3 0.0 0.0 0.0 2.900013340061364E-4 0.0 4 0.0 0.0 0.0 2.900013340061364E-4 0.0 5 0.0 0.0 0.0 0.0031900146740675007 0.0 6 0.0 0.0 0.0 0.003480016008073637 0.0 7 0.0 0.0 0.0 0.005510025346116592 0.0 8 0.0 0.0 0.0 0.00899004135419023 0.0 9 0.0 0.0 0.0 0.016820077372355913 0.0 10 0.0 0.0 0.0 0.017690081374374323 0.0 11 0.0 0.0 0.0 0.022040101384466368 0.0 12 0.0 0.0 0.0 0.02378010938850319 0.0 13 0.0 0.0 0.0 0.024070110722509322 0.0 14 0.0 0.0 0.0 0.024070110722509322 0.0 15 0.0 0.0 0.0 0.024070110722509322 0.0 16 0.0 0.0 0.0 0.024940114724527732 0.0 17 0.0 0.0 0.0 0.02523011605853387 0.0 18 0.0 0.0 0.0 0.027260125396576823 0.0 19 0.0 0.0 0.0 0.02929013473461978 0.0 20 0.0 0.0 0.0 0.031030142738656598 0.0 21 0.0 0.0 0.0 0.03132014407266273 0.0 22 0.0 0.0 0.0 0.0437902014349266 0.0 23 0.0 0.0 0.0 0.04785022011101251 0.0 24 0.0 0.0 0.0 0.051620237453092285 0.0 25 0.0 0.0 0.0 0.060320277473276375 0.0 26 0.0 0.0 0.0 0.0684403148254482 0.0 27 0.0 0.0 0.0 0.13543062298086572 0.0 28 0.0 0.0 0.0 0.18096083241982913 0.0 29 0.0 0.0 0.0 0.2610012006055228 0.0 30 0.0 0.0 0.0 0.3306015207669955 0.0 31 0.0 0.0 0.0 0.41412190496076284 0.0 32 0.0 0.0 0.0 0.5005423024945915 0.0 33 0.0 0.0 0.0 0.5860926960264017 0.0 34 0.0 0.0 0.0 0.675993109568304 0.0 35 0.0 0.0 0.0 0.8476738992999368 0.0 36 0.0 0.0 0.0 1.1208551559337172 0.0 37 0.0 0.0 0.0 1.6614176425211555 0.0 38 0.0 0.0 0.0 2.3350907414174107 0.0 39 0.0 0.0 0.0 3.003253814967549 0.0 40 0.0 0.0 0.0 3.8491877062634487 0.0 41 0.0 0.0 0.0 5.182323838689658 0.0 42 0.0 0.0 0.0 6.621890460696119 0.0 43 0.0 0.0 0.0 8.18615765632522 0.0 44 0.0 0.0 0.0 9.545973911479992 0.0 45 0.0 0.0 0.0 10.721059316872857 0.0 46 0.0 0.0 0.0 11.928914873008416 0.0 47 0.0 0.0 0.0 12.998149791489041 0.0 48 0.0 0.0 0.0 14.126834983440924 0.0 49 0.0 0.0 0.0 15.078619361649064 0.0 50 0.0 0.0 0.0 16.102904073358737 0.0 51 0.0 0.0 0.0 17.286109516103775 0.0 52 0.0 0.0 0.0 18.608515599171756 0.0 53 0.0 0.0 0.0 19.990951958379007 0.0 54 0.0 0.0 0.0 21.36990830157819 0.0 55 0.0 0.0 0.0 22.43479320004872 0.0 56 0.0 0.0 0.0 23.247376937933915 0.0 57 0.0 0.0 0.0 24.124050970634464 0.0 58 0.0 0.0 0.0 25.012905059363273 0.0 59 0.0 0.0 0.0 25.814468746556233 0.0 60 0.0 0.0 0.0 26.539762082905582 0.0 61 0.0 0.0 0.0 27.312615638031936 0.0 62 0.0 0.0 0.0 28.072129131794007 0.0 63 0.0 0.0 0.0 28.961853224524834 0.0 64 0.0 0.0 0.0 29.94089772812955 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACACT 15 0.0022225752 70.00797 34 CGGTTTA 15 0.0022238542 69.997826 62 GACGAAT 15 0.0022238542 69.997826 46 AGGGGGG 3085 0.0 59.33365 70 GCAGCGT 25 2.3890492E-4 55.998264 1 CGACGCT 20 0.0069448 52.50598 26 AAGCGAC 20 0.0069448 52.50598 31 AGCGACA 20 0.0069448 52.50598 32 CAGGTAT 20 0.0069487845 52.49837 1 ACATGTC 20 0.0069487845 52.49837 64 GTCTTAA 20 0.0069487845 52.49837 49 GCCGTAA 20 0.0069487845 52.49837 46 CGTCTAC 35 2.0847918E-5 49.998447 48 ACGTATG 35 2.0847918E-5 49.998447 40 CGAATGC 80 0.0 48.123505 41 ATGACGA 30 5.8745476E-4 46.66522 44 TATACCG 45 1.6826962E-6 46.665215 6 TGCCGTA 45 1.6826962E-6 46.665215 45 GCCGTCT 4300 0.0 46.393906 46 CCGTCTT 4285 0.0 46.311275 47 >>END_MODULE