FastQCFastQC Report
Wed 25 May 2016
SRR1780025_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780025_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302060
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3800212.580944183274845No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG8420.2787525657154208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAAAC252.3882066E-455.9999961
CTCTTCG252.3882066E-455.99999619
GTGGACC200.006947152.50000410
CCGTAAT200.006947152.50000429
TAACCGT352.0837884E-550.05
TCTTCGT350.001254340.020
AGCTACG350.001254340.016
GCCGTAT1700.039.11764554
TCGCCGT1750.038.00000452
ATCTCGG1850.035.94594642
AACCGTG400.002416619635.0000046
CCGTGCA400.002416619635.0000048
GATCAGT400.002416619635.0000041
GTCTACA400.002416619635.00000438
GTCACTA400.002416619635.00000423
ACCGTGC400.002416619635.0000047
CGCAAAG400.002416619635.00000412
CAATACC400.002416619635.0000044
GACATAC601.2135846E-535.07
GTCGCCG1950.034.10256651
Produced by FastQC (version 0.11.5)