FastQCFastQC Report
Wed 25 May 2016
SRR1780025_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780025_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302060
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT211066.9873535059259755No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG36321.2024101171952593No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC26350.8723432430642919No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15960.5283718466529829No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT11210.3711183208634046TruSeq Adapter, Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT9260.30656161027610407No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC6040.19996027279348474No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT4750.15725352578957824No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT4010.1327550817718334No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGT150.002223583669.9978419
GATAACT150.002223583669.9978442
GTCGGTC150.002223583669.9978416
GCCGTAT401.0639269E-861.2481246
TGCCGTA352.9692092E-759.99815445
AGGGGGG25350.059.781270
ATCGTAT601.8189894E-1258.33153539
CGTATGA1350.057.03528241
GTATGAC1300.056.53672442
TATGACG1350.054.4427743
CCTCGAC200.00694339752.5070829
CGCTCGA200.00694339752.5070832
CTCGACC200.00694339752.5070830
CGGTATC200.00694339752.5070825
TGACGTC1600.052.49838645
TGACGCT200.006947945352.49838618
TCGGAAA200.006947945352.49838611
CTCGAAA200.006947945352.49838639
TCGGTTC200.006947945352.49838667
ATATCGT608.367351E-1152.49838337