FastQCFastQC Report
Wed 25 May 2016
SRR1780024_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780024_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172685
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4444225.735877464747954No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG12570.7279149897211686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCC150.002221694770.052
GCGCGTC301.0191434E-770.06
TCTTCAG150.002221694770.016
TGTGCGC353.6889105E-970.03
CGTTCCC150.002221694770.053
TACGCGC150.002221694770.06
GGAACGA150.002221694770.022
CTTTGGT150.002221694770.013
GAACGAG150.002221694770.023
AATGTGA150.002221694770.022
CGAGTTC308.350093E-658.33333260
GAGTTCC308.350093E-658.33333261
TGCCGAG308.350093E-658.33333257
CAGCGAA252.3853306E-455.99999637
TGGCGTC252.3853306E-455.99999631
CCCGTAG252.3853306E-455.99999656
TGCTCTC252.3853306E-455.9999967
TTCCTGG252.3853306E-455.99999615
CTTGTGC200.006942084552.5000041
ATCTCGC200.006942084552.5000046
Produced by FastQC (version 0.11.5)