FastQCFastQC Report
Wed 25 May 2016
SRR1780024_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780024_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172685
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT2814816.300199785737036No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG41862.424066942699134No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC39242.272345600370617No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23511.3614384573066567No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT10250.5935663201783594RNA PCR Primer, Index 36 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC6860.3972551176998581No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT6180.3578770593855865No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT5310.30749630830703306No Hit
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC3500.20268118249992761No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT2860.1656194805570837No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAACCCATCTCGTATGCCGT2200.12739960042852594No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCAGT1770.10249876943567768No Hit
TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT1760.10191968034282074No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT150.00222173169.999718
CGTCTAC301.0191616E-769.9997148
CACGAAT150.00222173169.9997139
TGGCCGG207.9097415E-569.9997137
CTGCAAG301.0191616E-769.9997154
GTTCCGC150.00222173169.9997147
CGATGCG150.00222173169.9997141
AGGGGGG32200.064.1301770
CCGTCTA401.060107E-861.24974447
TGCCGAC700.059.9997545
CGTATGA650.059.23052641
CGAATGC650.059.23052641
GCCGACA308.350296E-658.3330946
AATCTCG1750.057.9997636
GATCGGG252.3853793E-455.9997730
CTTCCGC252.3853793E-455.9997723
AAACGAG252.3853793E-455.9997720
GCCGACT509.749783E-1055.9997746
ATCGGTT252.3853793E-455.9997722
CGACTTC509.749783E-1055.9997748
Produced by FastQC (version 0.11.5)