FastQCFastQC Report
Wed 25 May 2016
SRR1780023_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780023_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82784
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4710656.90229996134519No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG13751.660948975647468No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1890.22830498647081562No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC1450.17515461925009665No Hit
TCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGT1440.17394665635871664No Hit
CTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGG1380.16669887901043678No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1370.16549091611905684No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG830.10026091998453807No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGG150.002212811770.0380919
AGTGGGT150.002212811770.0380918
CACAAGT150.002212811770.0380914
ACAAGTG150.002212811770.0380915
TGCCGGC252.8086433E-670.03808619
CTTGTGC207.8876474E-569.995771
TGTTTCA150.002218126869.9957731
GAAATGA150.002218126869.9957752
TCTGAGT150.002218126869.9957742
ACACGTC150.002218126869.995776
GATGTTT150.002218126869.9957729
GATAAAG150.002218126869.9957730
GTACAGG150.002218126869.9957746
TTTACCT150.002218126869.9957741
TTTCGTC150.002218126869.9957739
TTTAACC150.002218126869.9957725
CTGAGTA150.002218126869.9957743
ATAATGC150.002218126869.9957721
CAGCAGT150.002218126869.9957770
GTGATAT150.002218126869.9957745
Produced by FastQC (version 0.11.5)