SRR1780023_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1780023_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82784
Sequences flagged as poor quality	0
Sequence length	76
%GC	48

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	29877	36.090307305759566	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	4865	5.8767394665635875	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC	3083	3.7241495941244684	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	2410	2.911190568225744	No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT	1509	1.822816003092385	RNA PCR Primer, Index 36 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC	1083	1.3082238113645148	No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC	510	0.6160610746037882	No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	497	0.6003575570158485	No Hit
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC	301	0.36359683030537304	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGACGT	263	0.31769424043293387	No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT	218	0.2633359103208349	RNA PCR Primer, Index 36 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	210	0.25367220718979516	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATATCGTATGCCGT	209	0.2524642442984152	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAACAATCTCGTATGCCGT	207	0.2500483185156552	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC	201	0.24280054116737532	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAGT	180	0.2174333204483958	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACACTCTCGTATGCCGT	161	0.19448202551217625	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGA	152	0.1836103594897565	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC	144	0.17394665635871664	RNA PCR Primer, Index 36 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	130	0.157035175879397	No Hit
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG	119	0.14374758407421723	No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	119	0.14374758407421723	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAAACTCGTATGCCGT	117	0.1413316582914573	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACACCAATCTCGTATGCCGT	116	0.1401236954000773	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGAATGCCGT	105	0.12683610359489758	No Hit
TCTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	102	0.12321221492075764	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACAACAATCTCGTATGCCGT	97	0.11717240046385775	No Hit
CTTATACACATCTCCGAGACCACGAGACTAACAACAATCTCGTATGCCGT	92	0.11113258600695787	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC	92	0.11113258600695787	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAG	91	0.1099246231155779	No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT	89	0.10750869733281794	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAAAAATCTCGTATGCCGT	88	0.10630073444143795	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTAAGCCGT	88	0.10630073444143795	No Hit
CTTATACACATCTCCGAGCCAACGAGACTAACAACAATCTCGTATGCCGT	83	0.10026091998453807	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCACGC	15	0.002214858	70.02177	68
TAAAACC	15	0.002214858	70.02177	27
ACGGGAC	20	7.8731246E-5	70.02177	54
AGAAACC	15	0.002214858	70.02177	30
GGGACCA	15	0.002214858	70.02177	56
TCAGCTA	15	0.002214858	70.02177	53
CGGGACC	20	7.8731246E-5	70.02177	55
TTACGGG	20	7.8731246E-5	70.02177	52
GGACCAT	15	0.002214858	70.02177	57
GGTTAAA	15	0.002214858	70.02177	24
TACGTTT	15	0.002214858	70.02177	27
CCGGGGC	20	7.8967554E-5	69.97946	7
ATGGCGA	20	7.8967554E-5	69.97946	17
TGGCGAT	20	7.8967554E-5	69.97946	18
CGTTCCG	20	7.8967554E-5	69.97946	39
GCCACTA	20	7.8967554E-5	69.97946	45
GCCGTTC	20	7.8967554E-5	69.97946	37
GGGGCGA	20	7.8967554E-5	69.97946	9
CCTTCAA	15	0.0022201783	69.979454	47
TTGTGGG	15	0.0022201783	69.979454	37

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content