##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780023_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82784 Sequences flagged as poor quality 0 Sequence length 76 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.90837601468883 32.0 27.0 32.0 22.0 32.0 2 30.451113741785854 32.0 32.0 32.0 27.0 32.0 3 30.81205305373019 32.0 32.0 32.0 27.0 32.0 4 30.556750096637032 32.0 32.0 32.0 27.0 32.0 5 30.84419694626981 32.0 32.0 32.0 27.0 32.0 6 33.26211586780054 37.0 32.0 37.0 22.0 37.0 7 34.95443563973715 37.0 37.0 37.0 32.0 37.0 8 32.85004348666409 37.0 32.0 37.0 22.0 37.0 9 34.85313587166602 37.0 37.0 37.0 27.0 37.0 10-11 34.872632392732896 37.0 37.0 37.0 29.5 37.0 12-13 35.09576125821415 37.0 37.0 37.0 32.0 37.0 14-15 34.95124057788945 37.0 37.0 37.0 27.0 37.0 16-17 33.74559697526092 37.0 34.5 37.0 24.5 37.0 18-19 34.0587311557789 37.0 37.0 37.0 27.0 37.0 20-21 34.9218327212988 37.0 37.0 37.0 29.5 37.0 22-23 34.451687524159254 37.0 37.0 37.0 27.0 37.0 24-25 34.64494950715114 37.0 37.0 37.0 29.5 37.0 26-27 34.91512248743719 37.0 37.0 37.0 29.5 37.0 28-29 35.134506667955165 37.0 37.0 37.0 32.0 37.0 30-31 34.31515751836103 37.0 34.5 37.0 29.5 37.0 32-33 34.661341563587165 37.0 37.0 37.0 29.5 37.0 34-35 34.25335813683804 37.0 34.5 37.0 27.0 37.0 36-37 33.779625289911095 37.0 34.5 37.0 24.5 37.0 38-39 33.432233281793586 37.0 34.5 37.0 22.0 37.0 40-41 34.1077925686123 37.0 37.0 37.0 24.5 37.0 42-43 33.93872608233475 37.0 37.0 37.0 24.5 37.0 44-45 34.183495603015075 37.0 37.0 37.0 27.0 37.0 46-47 33.53718713761113 37.0 34.5 37.0 22.0 37.0 48-49 32.453940374951685 37.0 32.0 37.0 17.5 37.0 50-51 33.23784789331272 37.0 32.0 37.0 22.0 37.0 52-53 32.83673777541554 37.0 32.0 37.0 22.0 37.0 54-55 33.17953348473135 37.0 32.0 37.0 22.0 37.0 56-57 32.263607701971395 37.0 32.0 37.0 17.5 37.0 58-59 32.67833760146888 37.0 32.0 37.0 17.5 37.0 60-61 32.51229102241979 37.0 32.0 37.0 17.5 37.0 62-63 33.81129203710862 37.0 32.0 37.0 27.0 37.0 64-65 34.08077043873212 37.0 34.5 37.0 27.0 37.0 66-67 34.0350067645922 37.0 37.0 37.0 27.0 37.0 68-69 33.53621472748357 37.0 32.0 37.0 22.0 37.0 70-71 30.592741350985698 34.5 27.0 37.0 13.0 37.0 72-73 31.048324555469655 37.0 29.5 37.0 13.0 37.0 74-75 32.29750314070351 37.0 32.0 37.0 13.0 37.0 76 32.55563877077696 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.5994257898883113 11101 2 -1.207691163527926 11101 3 -0.6798469930205329 11101 4 -0.7505816956978748 11101 5 0.3425293329176462 11101 6 0.6751262863162282 11101 7 0.8209932851949944 11101 8 0.46295729443594524 11101 9 -0.7267075784819141 11101 10-11 0.3043475500476944 11101 12-13 -0.8925605335033069 11101 14-15 -0.5433688235185699 11101 16-17 1.3323050883907612 11101 18-19 0.38453635749011994 11101 20-21 0.46430844302523155 11101 22-23 -0.6508117244139484 11101 24-25 -0.041492225241093195 11101 26-27 -0.4910320190261288 11101 28-29 -0.002094482983672208 11101 30-31 -0.37728107611160766 11101 32-33 -0.19586325549008166 11101 34-35 -0.0633736141111001 11101 36-37 0.18631202561509497 11101 38-39 -0.41569385422522487 11101 40-41 -0.14580411241213653 11101 42-43 -0.3021012273832753 11101 44-45 -0.8535851908426793 11101 46-47 0.024372186594021628 11101 48-49 0.6306929532714349 11101 50-51 -0.675760417040344 11101 52-53 -0.13314308509867345 11101 54-55 -0.8267730827025517 11101 56-57 -0.34995096172356455 11101 58-59 -1.2356646848001631 11101 60-61 -0.20133891254914715 11101 62-63 0.13606025204293104 11101 64-65 0.08466435137408013 11101 66-67 -1.0956869364926263 11101 68-69 -1.1616338499171093 11101 70-71 0.7600990895509661 11101 72-73 0.856309412209356 11101 74-75 -0.029347909385904813 11101 76 -0.43050854958975293 11102 1 -0.3270550233998968 11102 2 -0.217673338215981 11102 3 -1.2395617880294445 11102 4 -0.7726850825071452 11102 5 -0.740180114497683 11102 6 -2.3373514320438424 11102 7 0.19746387343029426 11102 8 -1.7823189978991714 11102 9 -1.3170819100327193 11102 10-11 -1.0190036085975862 11102 12-13 -0.11377265471542586 11102 14-15 -0.9998750623777539 11102 16-17 0.6790074056813111 11102 18-19 0.5601156801282698 11102 20-21 0.17981646441560883 11102 22-23 0.18929522745770555 11102 24-25 0.1759765804630078 11102 26-27 0.23071486154427845 11102 28-29 -0.34701426907992783 11102 30-31 -0.40027572851801807 11102 32-33 -0.7803552340997015 11102 34-35 0.4091754054967467 11102 36-37 -0.3940801312476552 11102 38-39 1.022095807093848 11102 40-41 -0.6554297808613327 11102 42-43 0.5907686478395462 11102 44-45 -1.3190040856733418 11102 46-47 -1.0665369043150719 11102 48-49 -0.563781200026245 11102 50-51 0.06612906602560997 11102 52-53 -0.7983837268099059 11102 54-55 -0.5691973251267939 11102 56-57 -1.1107352754490556 11102 58-59 1.3204850478201564 11102 60-61 0.47031884146154823 11102 62-63 0.23196043029604851 11102 64-65 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11103 44-45 -0.4550180716873342 11103 46-47 0.6109106481324815 11103 48-49 -0.18755742380246687 11103 50-51 0.11175843665498775 11103 52-53 0.7005673221411399 11103 54-55 -0.24664487757434728 11103 56-57 -0.6665045062182848 11103 58-59 -0.8097597074245968 11103 60-61 -0.43719864105593587 11103 62-63 0.435456933792544 11103 64-65 0.6778016062760415 11103 66-67 -0.6039825624353199 11103 68-69 -1.428602175708967 11103 70-71 -2.1054740627868895 11103 72-73 -0.4679741473683201 11103 74-75 1.1893549563757873 11103 76 1.5302757641357374 11104 1 -0.5864706078154818 11104 2 0.5121967916541479 11104 3 0.6062174327497765 11104 4 0.30263959281753117 11104 5 0.08027443095686237 11104 6 1.1285576588652475 11104 7 0.2653210162874373 11104 8 -1.1238774394576154 11104 9 0.04850250555170277 11104 10-11 -0.2488737384677222 11104 12-13 0.01220137125859111 11104 14-15 -1.1239010364037298 11104 16-17 -0.6237523345784339 11104 18-19 0.4237520437646296 11104 20-21 -0.21531340571425517 11104 22-23 -0.6261268504643667 11104 24-25 -0.16736757538114944 11104 26-27 0.5312018745312912 11104 28-29 -0.6018843989500624 11104 30-31 -0.7738146895569784 11104 32-33 -1.1879851042295684 11104 34-35 -0.030110308788962925 11104 36-37 -0.15122298839051496 11104 38-39 0.9878425603405958 11104 40-41 -0.0013713393028922383 11104 42-43 -0.19478330021240708 11104 44-45 0.809405005235746 11104 46-47 -0.6452706705488396 11104 48-49 0.465601916856869 11104 50-51 0.14129140368795134 11104 52-53 -0.9052019086280865 11104 54-55 0.04525072682125142 11104 56-57 -0.22144956116333958 11104 58-59 0.0631798530149652 11104 60-61 -1.3053305091878045 11104 62-63 -0.786383725548113 11104 64-65 -1.4650555365810973 11104 66-67 -0.4645594855122397 11104 68-69 0.9292274946207044 11104 70-71 0.9110094536966251 11104 72-73 0.020350028455855096 11104 74-75 -0.6925131754923441 11104 76 -1.0947242358642626 11105 1 -1.8906913870362594 11105 2 -0.544946065488709 11105 3 0.14225639378873822 11105 4 0.10004219022012961 11105 5 -1.0856346599522269 11105 6 -1.0282605229529338 11105 7 0.6520093279757475 11105 8 -1.3459553615355375 11105 9 0.5374635445127396 11105 10-11 -0.7826399722339517 11105 12-13 -0.1592272001699797 11105 14-15 -1.2816932441959352 11105 16-17 -0.6846289579550486 11105 18-19 -0.7398843198717273 11105 20-21 -0.34745626285711495 11105 22-23 -1.0925229543604758 11105 24-25 -1.1603870559006282 11105 26-27 -0.5601942293648108 11105 28-29 -0.6924688145344788 11105 30-31 -0.3820939103362022 11105 32-33 -0.6803552340997001 11105 34-35 -0.4908245945032519 11105 36-37 -0.08498922215675009 11105 38-39 -1.2142678292697866 11105 40-41 -0.8008843263158809 11105 42-43 -0.14559498852409547 11105 44-45 -1.8735495402188036 11105 46-47 -0.7483550861332517 11105 48-49 -2.172872109117158 11105 50-51 -1.7429618430652987 11105 52-53 -0.9983837268099052 11105 54-55 -1.0964700523995248 11105 56-57 -1.0925534572672362 11105 58-59 -0.6522422249071127 11105 60-61 -0.8478629767202719 11105 62-63 0.02286952120513064 11105 64-65 -1.154016575542137 11105 66-67 -0.5270594855122397 11105 68-69 -0.8803504274572163 11105 70-71 -0.6587957411085661 11105 72-73 -1.5609811403753149 11105 74-75 0.3522920193128485 11105 76 -0.8151787813188065 11106 1 -0.9078991792440512 11106 2 -0.4520889226315674 11106 3 0.4990745756069188 11106 4 0.5704967356746735 11106 5 0.2588458595282894 11106 6 -0.3535851982776066 11106 7 0.5688924448588608 11106 8 0.8404082748280999 11106 9 -0.13006892301972783 11106 10-11 0.1618405472465696 11106 12-13 -1.344941485884263 11106 14-15 0.32252753502483955 11106 16-17 -0.23089519172128092 11106 18-19 0.557680615193199 11106 20-21 0.498972308571453 11106 22-23 -0.4475554218929432 11106 24-25 -0.97093900395258 11106 26-27 0.9954875888170065 11106 28-29 1.0856156010499376 11106 30-31 -0.3184575466998396 11106 32-33 0.383443467199001 11106 34-35 0.6752468340681759 11106 36-37 0.45591986875234625 11106 38-39 0.782485417483457 11106 40-41 -0.010299910731461637 11106 42-43 0.3498595569304541 11106 44-45 -0.6370235661928234 11106 46-47 0.6493721865940216 11106 48-49 -0.9361837974288392 11106 50-51 0.7930771179736595 11106 52-53 0.8358695199433441 11106 54-55 0.848822155392682 11106 56-57 -0.5785924183061972 11106 58-59 1.6703227101578193 11106 60-61 -0.13568765204495037 11106 62-63 0.7046877030233176 11106 64-65 0.8117301777046109 11106 66-67 0.8657976573448991 11106 68-69 -0.4993439339507191 11106 70-71 1.0895808822680557 11106 72-73 2.413207171312994 11106 74-75 0.45034396736480176 11106 76 -0.5232956644356896 11107 1 -0.5385382769884153 11107 2 -1.2221390479448502 11107 3 -0.5952236700071154 11107 4 -0.2562576502902374 11107 5 0.18773057130773907 11107 6 -0.035916025345279934 11107 7 -2.3534133195521676 11107 8 -1.2078373392069892 11107 9 -0.26540726888438826 11107 10-11 0.02054982042952247 11107 12-13 -0.9013324633278756 11107 14-15 -1.603224344674409 11107 16-17 -1.5211520839518613 11107 18-19 -1.8920374299195828 11107 20-21 -1.0790352102255412 11107 22-23 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24-25 -0.25665328966685763 21611 26-27 0.5312018745312912 21611 28-29 0.04990131533566 21611 30-31 0.8333281675858757 21611 32-33 0.8120148957704316 21611 34-35 0.8806039769253147 21611 36-37 1.1344912973237768 21611 38-39 0.8360568460548805 21611 40-41 0.5164858035542537 21611 42-43 0.7873595569304541 21611 44-45 0.5951192909500307 21611 46-47 0.16722932945116042 21611 48-49 0.9566733454282996 21611 50-51 0.9716485465450901 21611 52-53 0.7108695199433441 21611 54-55 0.8756078696783973 21611 56-57 0.8410504388366533 21611 58-59 1.3399655673006805 21611 60-61 0.7303837765264802 21611 62-63 1.0082591315947482 21611 64-65 0.7045873205617568 21611 66-67 0.03544051448776031 21611 68-69 0.18815606604928092 21611 70-71 1.0627951679823404 21611 72-73 0.35070717131299745 21611 74-75 -0.5675131754923441 21611 76 1.0481329069928833 21612 1 0.09210082075594883 21612 2 0.44076822022557494 21612 3 -0.26878256725022354 21612 4 0.8383538785318159 21612 5 -0.93758271190028 21612 6 0.9857005160081016 21612 7 0.3903210162874373 21612 8 0.7689797033995305 21612 9 0.8520739341231263 21612 10-11 -0.0345880241819998 21612 12-13 0.8872013712585911 21612 14-15 -0.08818675068943804 21612 16-17 1.3673190939929967 21612 18-19 -1.2458908133782245 21612 20-21 -0.17067054857140818 21612 22-23 1.1506588638213415 21612 24-25 0.47548956747599647 21612 26-27 -0.0848695540401323 21612 28-29 0.5141870296213682 21612 30-31 0.735113881871591 21612 32-33 -0.4111993899438602 21612 34-35 -0.5211817373604006 21612 36-37 -0.017294416961945558 21612 38-39 -0.10144315394511239 21612 40-41 -0.20672848216003814 21612 42-43 1.0105738426447388 21612 44-45 0.4076192909500307 21612 46-47 1.3815150437368828 21612 48-49 1.465601916856869 21612 50-51 0.6680771179736595 21612 52-53 0.19301237708619823 21612 54-55 0.6702507268212514 21612 56-57 -0.16787813259191608 21612 58-59 -0.3207487184136042 21612 60-61 -0.16247336633065856 21612 62-63 -0.06316943983382117 21612 64-65 -0.009698393723958532 21612 66-67 0.2140119430591909 21612 68-69 0.5274417803349891 21612 70-71 -0.2764905463033749 21612 72-73 -0.2564356858298602 21612 74-75 -0.06751317549234415 21612 76 -0.862581378721405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 6.0 18 11.0 19 25.0 20 51.0 21 102.0 22 240.0 23 383.0 24 590.0 25 776.0 26 1103.0 27 1477.0 28 1940.0 29 2559.0 30 3456.0 31 4981.0 32 7224.0 33 11259.0 34 17801.0 35 21947.0 36 6852.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.82723879244736 10.77809589388613 13.978182872467654 56.41648244119886 2 11.214196837362133 9.147247490305514 61.46607231302625 18.172483359306106 3 10.637321221492076 13.140220332431388 62.81527831465017 13.407180131426363 4 8.578952454580595 51.30459992269037 26.952068032470045 13.164379590258987 5 8.41950135291844 23.597555083107846 60.60470622342482 7.378237340548899 6 13.237337070100628 63.071236153223566 14.604800618499414 9.086626158176394 7 12.595581218366089 22.121691651667614 13.935228247345469 51.347498882620826 8 11.716032083494394 63.02546385775029 9.219172787011983 16.039331271743333 9 11.393505991495942 16.512852725164283 7.892829532276768 64.200811751063 10-11 12.168634675203092 37.82623138948449 32.69169208467974 17.31344185063268 12-13 13.212094124468496 11.668921530730577 38.04358330112099 37.07540104367994 14-15 13.601662156938538 9.776043679938153 14.889954580595285 61.73233958252803 16-17 38.44885485117897 31.87210088906069 8.025705450328566 21.653338809431773 18-19 40.773356526781185 17.776207388079534 8.120122731994877 33.330313353144405 20-21 15.876256281407036 10.494177618863548 8.157977386934673 65.47158871279476 22-23 13.60528604561268 36.39773386161577 8.235890993428683 41.76108909934287 24-25 37.96660948561212 36.434801517311364 8.483534272391214 17.1150547246853 26-27 40.90348927121593 40.04144113667118 9.893316257490818 9.161753334622077 28-29 14.634779877253177 18.478470980524815 31.29258686512347 35.59416227709853 30-31 11.148762926452111 60.14303662897458 15.782956412486712 12.925244032086594 32-33 11.031241694251818 44.7145238843115 8.324473868606084 35.9297605528306 34-35 10.957894673262423 44.778057634346254 9.895446391360284 34.368601301031035 36-37 10.825763432547351 60.9441437959026 10.771405102435253 17.458687669114806 38-39 12.287398531117125 20.057015848473135 35.39633262466177 32.25925299574797 40-41 11.314931142788113 10.239188209712491 39.888862043971976 38.55701860352742 42-43 37.424272624591545 9.514921992498234 36.980327492585815 16.080477890324413 44-45 14.45146405102435 34.22823250869733 39.90807402396598 11.41222941631233 46-47 37.15479958443065 12.165068013240232 18.466259150982147 32.213873251346975 48-49 35.60229029764206 9.170250289911094 12.298270197139544 42.929189215307304 50-51 16.66445206803247 9.36533629686896 37.93969849246231 36.03051314263625 52-53 10.811871859296481 9.761548125241593 62.29283436412834 17.13374565133359 54-55 10.151002657646774 9.603769026334863 44.038415076105345 36.206813239913025 56-57 36.13681387707769 9.325473521453421 19.15889543873212 35.37881716273676 58-59 13.49323797846059 12.700157649631244 56.89218818896211 16.914416182946056 60-61 35.18131522999614 35.64154909161191 19.473569771936607 9.703565906455355 62-63 12.749752361624509 68.42671112077505 8.687879007513711 10.135657510086734 64-65 9.447353955626431 72.11520456179137 8.213882294668045 10.223559187914152 66-67 9.601783007072836 72.2462144319685 7.69133200050736 10.460670560451309 68-69 12.227000386548125 69.92957576343255 7.740626207962892 10.102797642056437 70-71 32.86202647854658 49.23717143409355 7.671772323154233 10.229029764205643 72-73 68.90028448728867 13.345534274376211 7.57968362114266 10.174497617192454 74-75 72.57022047719722 10.106916339474479 7.121111446692842 10.201751736635458 76 72.4569923649367 10.066927611868175 7.2835121291195515 10.192567894075577 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.5 11 1.5 12 2.0 13 1.5 14 1.5 15 2.5 16 4.5 17 6.0 18 7.0 19 15.0 20 32.0 21 42.0 22 58.0 23 80.5 24 84.5 25 82.0 26 107.5 27 154.0 28 224.0 29 273.0 30 286.5 31 290.5 32 406.5 33 532.0 34 559.5 35 629.0 36 772.0 37 873.0 38 925.5 39 1144.0 40 1462.0 41 1916.0 42 2218.0 43 3662.0 44 10207.0 45 22790.5 46 30273.0 47 16775.5 48 3131.0 49 2248.0 50 1512.0 51 1506.5 52 1415.0 53 1211.5 54 1094.0 55 1068.5 56 1044.0 57 965.0 58 885.0 59 805.5 60 625.0 61 577.0 62 630.0 63 566.5 64 472.5 65 412.5 66 323.0 67 263.0 68 247.0 69 213.0 70 169.0 71 143.0 72 129.5 73 114.5 74 99.0 75 85.0 76 72.5 77 50.5 78 33.0 79 25.0 80 25.5 81 20.0 82 19.5 83 25.0 84 18.5 85 9.5 86 8.0 87 9.0 88 8.5 89 6.0 90 5.0 91 7.5 92 9.0 93 8.0 94 8.0 95 14.0 96 19.0 97 38.5 98 109.0 99 1228.0 100 2296.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006039814456899884 2 0.006039814456899884 3 0.0 4 0.0 5 0.0 6 0.0036238886741399305 7 0.0012079628913799767 8 0.0 9 0.0 10-11 0.0018119443370699653 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0024159257827599534 20-21 0.0 22-23 0.0 24-25 0.007247777348279861 26-27 0.019327406262079627 28-29 0.014495554696559722 30-31 0.009663703131039814 32-33 0.012079628913799769 34-35 0.004227870119829919 36-37 0.0 38-39 0.0 40-41 0.004831851565519907 42-43 0.004227870119829919 44-45 0.0 46-47 0.007247777348279861 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.004831851565519907 56-57 0.0 58-59 0.006643795902589872 60-61 0.0 62-63 0.0024159257827599534 64-65 0.01147564746810978 66-67 0.003019907228449942 68-69 0.0 70-71 0.0 72-73 0.004227870119829919 74-75 0.01087166602241979 76 0.009663703131039814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 82784.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.667858523386162 #Duplication Level Percentage of deduplicated Percentage of total 1 69.63245761615116 12.99888867413993 2 8.185583020577198 3.0561461151913414 3 4.316034683577068 2.4171337456513333 4 3.546007506147276 2.6478546579049094 5 3.222466675294422 3.0078275995361423 6 2.8148052284198264 3.152783146501739 7 2.225960916267633 2.9087746424429843 8 1.695353953668953 2.531890220332431 9 1.3524006729649283 2.272178198685736 >10 2.7371554290151416 6.870892926169309 >50 0.10353306587291317 1.4966660224197914 >100 0.12294551572408438 4.289476227290297 >500 0.006470816617057073 0.6160610746037882 >1k 0.03235408308528536 15.6431194433707 >5k 0.0 0.0 >10k+ 0.006470816617057073 36.090307305759566 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT 29877 36.090307305759566 No Hit TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG 4865 5.8767394665635875 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC 3083 3.7241495941244684 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 2410 2.911190568225744 No Hit ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT 1509 1.822816003092385 RNA PCR Primer, Index 36 (95% over 23bp) TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC 1083 1.3082238113645148 No Hit CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC 510 0.6160610746037882 No Hit ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT 497 0.6003575570158485 No Hit TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC 301 0.36359683030537304 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGACGT 263 0.31769424043293387 No Hit TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT 218 0.2633359103208349 RNA PCR Primer, Index 36 (95% over 22bp) CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG 210 0.25367220718979516 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACAATATCGTATGCCGT 209 0.2524642442984152 No Hit CTTATACACATCTCCGAGCCCACGAGACTACCAACAATCTCGTATGCCGT 207 0.2500483185156552 No Hit TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC 201 0.24280054116737532 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAGT 180 0.2174333204483958 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACACTCTCGTATGCCGT 161 0.19448202551217625 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGA 152 0.1836103594897565 No Hit CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC 144 0.17394665635871664 RNA PCR Primer, Index 36 (95% over 21bp) CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG 130 0.157035175879397 No Hit CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG 119 0.14374758407421723 No Hit CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT 119 0.14374758407421723 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACAAACTCGTATGCCGT 117 0.1413316582914573 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACACCAATCTCGTATGCCGT 116 0.1401236954000773 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGAATGCCGT 105 0.12683610359489758 No Hit TCTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT 102 0.12321221492075764 No Hit CTTATACACATCTCCGAGCCCACGAGACAAACAACAATCTCGTATGCCGT 97 0.11717240046385775 No Hit CTTATACACATCTCCGAGACCACGAGACTAACAACAATCTCGTATGCCGT 92 0.11113258600695787 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC 92 0.11113258600695787 No Hit TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAG 91 0.1099246231155779 No Hit CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT 89 0.10750869733281794 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAAAAATCTCGTATGCCGT 88 0.10630073444143795 No Hit CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTAAGCCGT 88 0.10630073444143795 No Hit CTTATACACATCTCCGAGCCAACGAGACTAACAACAATCTCGTATGCCGT 83 0.10026091998453807 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0012079628913799767 0.0 2 0.0 0.0 0.0 0.015703517587939697 0.0 3 0.0 0.0 0.0 0.015703517587939697 0.0 4 0.0 0.0 0.0 0.04227870119829919 0.0 5 0.0 0.0 0.0 0.059190181677618865 0.0 6 0.0 0.0 0.0 0.059190181677618865 0.0 7 0.0 0.0 0.0 0.059190181677618865 0.0 8 0.0 0.0 0.0 0.059190181677618865 0.0 9 0.0 0.0 0.0 0.06039814456899884 0.0 10 0.0 0.0 0.0 0.06885388480865867 0.0 11 0.0 0.0 0.0 0.07006184770003865 0.0 12 0.0 0.0 0.0 0.07006184770003865 0.0 13 0.0 0.0 0.0 0.07006184770003865 0.0 14 0.0 0.0 0.0 0.09180517974487824 0.0 15 0.0 0.0 0.0 0.10267684576729803 0.0 16 0.0 0.0 0.0 0.12200425202937766 0.0 17 0.0 0.0 0.0 0.1292520293776575 0.0 18 0.0 0.0 0.0 0.1292520293776575 0.0 19 0.0 0.0 0.0 0.13891573250869732 0.0 20 0.0 0.0 0.0 0.1509953614224971 0.0 21 0.0 0.0 0.0 0.1509953614224971 0.0 22 0.0 0.0 0.0 0.1824023965983765 0.0 23 0.0 0.0 0.0 0.21501739466563588 0.0 24 0.0 0.0 0.0 0.22226517201391574 0.0 25 0.0 0.0 0.0 0.23676072671047546 0.0 26 0.0 0.0 0.0 0.25246424429841513 0.0 27 0.0 0.0 0.0 0.31648627754155395 0.0 28 0.0 0.0 0.0 0.35272516428295325 0.0 29 0.0 0.0 0.0 0.41070738306919213 0.0 30 0.0 0.0 0.0 0.4396984924623116 0.0 31 0.0 0.0 0.0 0.5085523772709702 0.0 32 0.0 0.0 0.0 0.570158484731349 0.0 33 0.0 0.0 0.0 0.6861229223038269 0.0 34 0.0 0.0 0.0 0.8818129107073831 0.0 35 0.0 0.0 0.0 1.0231445689988403 0.0 36 0.0 0.0 0.0 1.2816486277541554 0.0 37 0.0 0.0 0.0 1.774497487437186 0.0 38 0.0 0.0 0.0 2.138094317742559 0.0 39 0.0 0.0 0.0 2.6780537301894087 0.0 40 0.0 0.0 0.0 3.175734441437959 0.0 41 0.0 0.0 0.0 3.695158484731349 0.0 42 0.0 0.0 0.0 4.323299188248937 0.0 43 0.0 0.0 0.0 4.932112485504446 0.0 44 0.0 0.0 0.0 5.501063007344414 0.0 45 0.0 0.0 0.0 5.990287978353305 0.0 46 0.0 0.0 0.0 6.634132199458833 0.0 47 0.0 0.0 0.0 7.170467723231543 0.0 48 0.0 0.0 0.0 7.701971395438732 0.0 49 0.0 0.0 0.0 8.105431001159644 0.0 50 0.0 0.0 0.0 8.597071897951295 0.0 51 0.0 0.0 0.0 9.111664089679165 0.0 52 0.0 0.0 0.0 9.490964437572478 0.0 53 0.0 0.0 0.0 10.030923850019327 0.0 54 0.0 0.0 0.0 10.363113645148822 0.0 55 0.0 0.0 0.0 10.694095477386934 0.0 56 0.0 0.0 0.0 11.072187862388867 0.0 57 0.0 0.0 0.0 11.347603401623502 0.0 58 0.0 0.0 0.0 11.589195979899497 0.0 59 0.0 0.0 0.0 11.889978739853111 0.0 60 0.0 0.0 0.0 12.228208349439505 0.0 61 0.0 0.0 0.0 12.5 0.0 62 0.0 0.0 0.0 12.770583687669115 0.0 63 0.0 0.0 0.0 13.088277928102048 0.0 64 0.0 0.0 0.0 13.373357170467724 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCACGC 15 0.002214858 70.02177 68 TAAAACC 15 0.002214858 70.02177 27 ACGGGAC 20 7.8731246E-5 70.02177 54 AGAAACC 15 0.002214858 70.02177 30 GGGACCA 15 0.002214858 70.02177 56 TCAGCTA 15 0.002214858 70.02177 53 CGGGACC 20 7.8731246E-5 70.02177 55 TTACGGG 20 7.8731246E-5 70.02177 52 GGACCAT 15 0.002214858 70.02177 57 GGTTAAA 15 0.002214858 70.02177 24 TACGTTT 15 0.002214858 70.02177 27 CCGGGGC 20 7.8967554E-5 69.97946 7 ATGGCGA 20 7.8967554E-5 69.97946 17 TGGCGAT 20 7.8967554E-5 69.97946 18 CGTTCCG 20 7.8967554E-5 69.97946 39 GCCACTA 20 7.8967554E-5 69.97946 45 GCCGTTC 20 7.8967554E-5 69.97946 37 GGGGCGA 20 7.8967554E-5 69.97946 9 CCTTCAA 15 0.0022201783 69.979454 47 TTGTGGG 15 0.0022201783 69.979454 37 >>END_MODULE