FastQCFastQC Report
Wed 25 May 2016
SRR1780022_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780022_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59302
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3588360.50892044113183No Hit
CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG8041.3557721493372905No Hit
ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC910.15345182287275302No Hit
CTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGG840.14164783649792587No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG840.14164783649792587No Hit
TCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGT670.1129810124447742No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTGA150.002214485570.06
TGTGCGC150.002214485570.03
GACTAAT150.002214485570.026
GCGCGTG200.00691973152.56
GGTTTTG200.00691973152.517
CCCGCCG200.00691973152.514
TCTGCGG200.00691973152.53
TCTGCCT200.00691973152.59
AAAATAC200.00691973152.525
GTGCGCG200.00691973152.54
GTAATAT200.00691973152.523
AAAAAGG1300.051.15384770
GTATCAT2000.050.74999657
CGTATCA2000.050.74999656
TATCATT2050.049.51219658
ATCATTA2200.049.3181859
CCGTATC2100.048.33333655
GCCGTAT2100.048.33333654
TCGGTGG2250.048.2222245
CGGTGGT2400.048.12546
Produced by FastQC (version 0.11.5)