##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780022_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59302 Sequences flagged as poor quality 0 Sequence length 76 %GC 82 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.943087922835655 32.0 27.0 32.0 22.0 32.0 2 29.841539914336785 32.0 32.0 32.0 22.0 32.0 3 29.808927186266907 32.0 32.0 32.0 22.0 32.0 4 29.347779164277764 32.0 32.0 32.0 22.0 32.0 5 29.958382516609895 32.0 32.0 32.0 27.0 32.0 6 34.08694479106944 37.0 37.0 37.0 27.0 37.0 7 33.74415702674446 37.0 37.0 37.0 22.0 37.0 8 34.08171731138916 37.0 37.0 37.0 27.0 37.0 9 34.09790563556035 37.0 37.0 37.0 27.0 37.0 10-11 34.05502343934437 37.0 37.0 37.0 27.0 37.0 12-13 33.74056524231898 37.0 37.0 37.0 22.0 37.0 14-15 34.526955246028805 37.0 37.0 37.0 29.5 37.0 16-17 34.47669555832856 37.0 37.0 37.0 29.5 37.0 18-19 33.97650163569526 37.0 37.0 37.0 24.5 37.0 20-21 34.4160146369431 37.0 37.0 37.0 29.5 37.0 22-23 34.46352568210179 37.0 37.0 37.0 29.5 37.0 24-25 34.03139860375704 37.0 37.0 37.0 24.5 37.0 26-27 34.44216889818219 37.0 37.0 37.0 29.5 37.0 28-29 34.32033489595629 37.0 37.0 37.0 27.0 37.0 30-31 34.24720920036424 37.0 37.0 37.0 27.0 37.0 32-33 34.289400020235405 37.0 37.0 37.0 27.0 37.0 34-35 34.31553741863681 37.0 37.0 37.0 27.0 37.0 36-37 34.851573302755384 37.0 37.0 37.0 29.5 37.0 38-39 34.955633874068326 37.0 37.0 37.0 29.5 37.0 40-41 34.51405517520489 37.0 37.0 37.0 29.5 37.0 42-43 34.47692320663721 37.0 37.0 37.0 27.0 37.0 44-45 34.277629759535934 37.0 37.0 37.0 27.0 37.0 46-47 34.45439445549897 37.0 37.0 37.0 29.5 37.0 48-49 34.89591413443054 37.0 37.0 37.0 32.0 37.0 50-51 35.11363866311423 37.0 37.0 37.0 32.0 37.0 52-53 34.414758355536065 37.0 37.0 37.0 27.0 37.0 54-55 34.55086674985667 37.0 37.0 37.0 29.5 37.0 56-57 34.38194327341405 37.0 37.0 37.0 27.0 37.0 58-59 34.11618495160366 37.0 37.0 37.0 24.5 37.0 60-61 33.9361657279687 37.0 37.0 37.0 24.5 37.0 62-63 33.67059289737277 37.0 37.0 37.0 22.0 37.0 64-65 33.48995818016256 37.0 37.0 37.0 17.5 37.0 66-67 33.5515412633638 37.0 37.0 37.0 22.0 37.0 68-69 33.69870324778253 37.0 37.0 37.0 22.0 37.0 70-71 33.72650163569526 37.0 37.0 37.0 22.0 37.0 72-73 33.97149337290479 37.0 37.0 37.0 24.5 37.0 74-75 34.10443155374186 37.0 37.0 37.0 27.0 37.0 76 34.346666216991 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 0.03946533815748765 11101 2 -0.04141513184865531 11101 3 -0.2502369694460249 11101 4 0.18183861712306992 11101 5 0.9154130382234662 11101 6 -0.8767765445634694 11101 7 -0.5760677837774608 11101 8 -0.6281025823790642 11101 9 -2.2463096634337454 11101 10-11 -0.21403406322590968 11101 12-13 -0.6211287605433 11101 14-15 -0.24829962793141647 11101 16-17 -1.447812865278891 11101 18-19 0.28760116729610274 11101 20-21 -1.5921276523970462 11101 22-23 -1.0159463070726318 11101 24-25 -0.4159300360704492 11101 26-27 0.14559530122951259 11101 28-29 -0.549516470388113 11101 30-31 -1.0699041162647234 11101 32-33 -0.22357821269793732 11101 34-35 -0.6918728500028166 11101 36-37 -0.17101297286081518 11101 38-39 -1.5695037088138548 11101 40-41 -0.0807066769146445 11101 42-43 -0.6066285221755336 11101 44-45 -0.525914194640329 11101 46-47 -1.0636566622861565 11101 48-49 -3.504740255399284 11101 50-51 -0.7780569485282172 11101 52-53 -0.013551860394059645 11101 54-55 -1.1400897556984546 11101 56-57 -1.3574233466450707 11101 58-59 0.3579839115504697 11101 60-61 -0.27503067881323773 11101 62-63 0.6664757663003016 11101 64-65 0.14138623015972485 11101 66-67 -1.1734779522192298 11101 68-69 -1.940305220423987 11101 70-71 -0.43976348954361555 11101 72-73 -1.1601558493277224 11101 74-75 -2.6540630722861103 11101 76 -0.6880373317687045 11102 1 -0.6846725928769963 11102 2 0.5700791210249072 11102 3 -1.0939151303655663 11102 4 0.7531029849391615 11102 5 -0.29608121465009773 11102 6 1.8508096623330843 11102 7 0.4618632507052993 11102 8 0.2822422452071436 11102 9 -1.0796429967670775 11102 10-11 0.0014831781533999333 11102 12-13 0.35013560727279724 11102 14-15 -0.5678398578164732 11102 16-17 -0.36333010665820353 11102 18-19 -0.0836632005199931 11102 20-21 0.019941313120192206 11102 22-23 -0.19180837603814638 11102 24-25 -0.933171415380798 11102 26-27 0.5116872552525038 11102 28-29 0.42462146064637096 11102 30-31 -0.1842719323566797 11102 32-33 -0.16955522419218738 11102 34-35 -0.2953211258648807 11102 36-37 0.43645829150700166 11102 38-39 -0.8677795708828171 11102 40-41 0.7600979207864995 11102 42-43 0.3870496387440028 11102 44-45 -0.5006268383184889 11102 46-47 -0.10331183469994443 11102 48-49 0.4751448020719877 11102 50-51 0.36849477560971877 11102 52-53 -0.09171277993428362 11102 54-55 1.0449677155659103 11102 56-57 -0.3223658753807044 11102 58-59 -0.22879769764492863 11102 60-61 0.19623368900285243 11102 62-63 0.3842918582543291 11102 64-65 -0.6827517008747535 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46-47 0.13473043448804134 11103 48-49 0.43343178761147527 11103 50-51 0.47194305147178284 11103 52-53 0.3982760965951826 11103 54-55 2.2513080937639103 11103 56-57 1.2872003092689113 11103 58-59 1.9069086427332635 11103 60-61 0.606689751294283 11103 62-63 2.704647809311055 11103 64-65 1.4768701011274743 11103 66-67 1.9937263488560362 11103 68-69 1.0688345645222483 11103 70-71 -0.12524736051135932 11103 72-73 1.701134473252921 11103 74-75 0.977657357821414 11103 76 0.7130379370484974 11104 1 -0.16743121356665114 11104 2 -1.947162258285438 11104 3 -1.5766737510552211 11104 4 -3.833103911612561 11104 5 -2.6409088008569945 11104 6 -4.459535165253126 11104 7 -1.572619507915391 11104 8 -3.7177577547928564 11104 9 -3.5624016174567323 11104 10-11 -3.481275442536255 11104 12-13 -2.3740023237616867 11104 14-15 -3.636805375057854 11104 16-17 -2.1564335549340683 11104 18-19 -2.3078011315544735 11104 20-21 -1.2731621351556655 11104 22-23 -2.760773893279527 11104 24-25 -3.415930036070449 11104 26-27 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0.09999902307442454 21612 34-35 -0.3991899231735445 21612 36-37 -0.5193869566006555 21612 38-39 0.5926914131373735 21612 40-41 0.2310819409715279 21612 42-43 1.1376804209138953 21612 44-45 0.11880125251413887 21612 46-47 1.6351238255187255 21612 48-49 -0.22628497084643584 21612 50-51 0.9597479295205602 21612 52-53 1.2043343184677298 21612 54-55 0.2981216264153659 21612 56-57 0.24786120620046148 21612 58-59 0.2770896026073828 21612 60-61 0.38757094720301666 21612 62-63 -0.6989713881712376 21612 64-65 -0.09276011130368289 21612 66-67 1.253757820138496 21612 68-69 0.6722964055922702 21612 70-71 0.8224316324076071 21612 72-73 0.18618561408691647 21612 74-75 0.16097757812039504 21612 76 1.672125269857311 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 9.0 13 30.0 14 54.0 15 94.0 16 157.0 17 266.0 18 339.0 19 330.0 20 376.0 21 407.0 22 460.0 23 571.0 24 609.0 25 689.0 26 761.0 27 889.0 28 1045.0 29 1223.0 30 1403.0 31 1716.0 32 2177.0 33 3118.0 34 5207.0 35 10194.0 36 27176.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 79.56392701763852 6.068935280428991 3.726687126909716 10.640450575022765 2 71.53215743145257 7.45843310512293 13.446426764695962 7.562982698728542 3 71.44230282794557 7.268005598556517 12.529299674541743 8.760391898956172 4 70.02124717547468 7.837846952885232 11.12610030015851 11.014805571481569 5 69.92344271694041 10.508920441131833 13.69093791103167 5.876698930896091 6 75.85032292878704 13.15323518996307 6.062292372809902 4.934149508439992 7 73.70533380550074 9.36071904352372 7.128041685637679 9.805905465337853 8 73.83686615740038 13.087469014013253 6.305121330163066 6.770543498423298 9 72.31122053219116 6.50231020876193 6.229132238373073 14.957337020673839 10-11 73.80948365991028 9.195305385990354 8.987892482546963 8.007318471552392 12-13 72.99961214819312 8.004924031635218 9.609450093590327 9.38601372658134 14-15 74.6766496349134 8.434933643614778 7.637307971197788 9.251108750274026 16-17 74.52109540993558 8.643890593909143 7.14562746619001 9.689386529965264 18-19 72.85290298645891 8.563936527208647 8.886022158142358 9.697138328190082 20-21 74.33389544688028 8.354974704890388 8.042158516020235 9.268971332209107 22-23 74.34719189227928 8.4087249057781 7.453437096869388 9.790646105073227 24-25 73.32591355963643 9.865769548574223 7.270535066862278 9.537781824927068 26-27 74.43570350508858 9.177986323662088 7.878649904299362 8.507660266949975 28-29 73.42979283130549 8.524523815546505 9.063314193205677 8.982369159942326 30-31 73.53204047217538 8.572512647554806 8.023608768971332 9.871838111298482 32-33 73.13478143550999 8.585739341608203 9.853109821910415 8.426369400971398 34-35 73.21118399973018 9.131688561358539 8.888851414020472 8.768276024890808 36-37 74.17812665997758 8.209880186507702 9.219146550197722 8.392846603317004 38-39 74.76476341438737 7.660787157262824 9.787190988499544 7.787258439850259 40-41 74.21544324716278 8.783157113708032 9.165949983980035 7.835449655149154 42-43 72.97684693344125 8.985514578169001 9.615352186303772 8.422286302085968 44-45 72.61260349741151 7.59262069779599 10.630512133016307 9.164263671776194 46-47 73.5639064779137 7.337082535854911 9.94409922177348 9.154911764457898 48-49 73.77407170078581 7.636336042629253 10.562881521702472 8.026710734882466 50-51 74.25610671253551 7.677973676000641 9.67967689440889 8.38624271705495 52-53 71.79258010118043 8.204890387858347 10.549747048903878 9.452782462057336 54-55 72.69101701489014 7.981315660781436 9.504898736952159 9.822768587376267 56-57 72.87145242070116 8.40036424343603 8.941670460869124 9.786512874993676 58-59 72.4226608544616 8.94849115943373 9.77816375915886 8.850684226945809 60-61 72.22161328128294 8.572295810392655 9.933138284866319 9.272952623458092 62-63 72.08974856237036 9.290207254759776 9.557496753739523 9.062547429130339 64-65 72.59152459486349 9.380954789969815 9.278089745535487 8.749430869631203 66-67 72.49523584667014 10.459213788218628 7.798876840312325 9.246673524798894 68-69 72.5137224812607 10.511715752818274 7.744454093978971 9.230107671942058 70-71 72.53564472474937 10.193843222232529 7.768062663889849 9.502449389128254 72-73 72.90426482740013 9.823099883640534 7.464712727027437 9.807922561931905 74-75 73.2558727803167 9.27924585574799 8.057471458203343 9.407409905731969 76 75.26096561493448 7.489165078669117 8.121553483195331 9.128315823201067 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 4.0 16 6.0 17 5.0 18 12.0 19 21.0 20 36.0 21 49.0 22 46.0 23 69.5 24 81.5 25 67.0 26 78.0 27 102.5 28 146.0 29 176.0 30 174.5 31 174.0 32 217.0 33 259.0 34 311.0 35 402.5 36 474.5 37 507.0 38 548.5 39 607.0 40 696.0 41 794.5 42 821.0 43 879.0 44 1262.0 45 1478.5 46 1370.0 47 1223.0 48 962.0 49 868.0 50 888.0 51 856.0 52 787.0 53 754.5 54 759.0 55 760.0 56 762.5 57 707.5 58 651.0 59 602.0 60 528.5 61 479.0 62 454.0 63 406.5 64 334.0 65 279.0 66 248.0 67 247.0 68 200.0 69 158.5 70 129.5 71 95.0 72 97.5 73 96.0 74 84.0 75 76.0 76 60.5 77 49.0 78 50.5 79 48.0 80 41.5 81 39.5 82 42.0 83 40.0 84 46.0 85 58.0 86 64.0 87 64.0 88 63.0 89 79.5 90 93.0 91 98.0 92 107.0 93 142.0 94 194.5 95 260.5 96 309.0 97 378.5 98 716.5 99 18686.0 100 36387.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0016862837678324508 4 0.0 5 0.0 6 0.0016862837678324508 7 0.0016862837678324508 8 0.0016862837678324508 9 0.0 10-11 0.0 12-13 0.0016862837678324508 14-15 0.0016862837678324508 16-17 0.0 18-19 0.0016862837678324508 20-21 0.0033725675356649017 22-23 8.431418839162254E-4 24-25 0.0016862837678324508 26-27 0.0042157094195811266 28-29 0.0042157094195811266 30-31 0.0033725675356649017 32-33 0.010117702606994705 34-35 0.005058851303497353 36-37 0.0025294256517486764 38-39 0.0 40-41 0.0016862837678324508 42-43 0.0016862837678324508 44-45 0.0016862837678324508 46-47 8.431418839162254E-4 48-49 0.0 50-51 0.0042157094195811266 52-53 0.0033725675356649017 54-55 0.0016862837678324508 56-57 0.0016862837678324508 58-59 0.0025294256517486764 60-61 8.431418839162254E-4 62-63 0.005058851303497353 64-65 0.0016862837678324508 66-67 0.008431418839162253 68-69 0.0025294256517486764 70-71 0.0025294256517486764 72-73 0.005058851303497353 74-75 0.005058851303497353 76 0.005058851303497353 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 59302.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.06681056288152 #Duplication Level Percentage of deduplicated Percentage of total 1 74.22835411691872 14.152979663417762 2 6.358892721323074 2.4248760581430644 3 4.227469709029805 2.4181309230717347 4 3.8294861590165383 2.9206434858858046 5 3.1661802423277616 3.018447944420087 6 2.900857875652251 3.318606455094263 7 1.7688157778367384 2.360797274965431 8 1.2204828867073494 1.8616572796870259 9 0.74290262669143 1.2748305284813328 >10 1.494649332272044 3.745236248355873 >50 0.04422039444591846 0.6391015480084989 >100 0.0 0.0 >500 0.008844078889183692 1.3557721493372905 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.008844078889183692 60.50892044113183 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 35883 60.50892044113183 No Hit CTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTG 804 1.3557721493372905 No Hit ATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTC 91 0.15345182287275302 No Hit CTCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGG 84 0.14164783649792587 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 84 0.14164783649792587 No Hit TCTTATACACATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGT 67 0.1129810124447742 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006745135071329803 0.0 2 0.0 0.0 0.0 0.02192168898182186 0.0 3 0.0 0.0 0.0 0.02192168898182186 0.0 4 0.0 0.0 0.0 0.047215945499308624 0.0 5 0.0 0.0 0.0 0.08768675592728745 0.0 6 0.0 0.0 0.0 0.08768675592728745 0.0 7 0.0 0.0 0.0 0.08768675592728745 0.0 8 0.0 0.0 0.0 0.09780445853428214 0.0 9 0.0 0.0 0.0 0.1028633098377795 0.0 10 0.0 0.0 0.0 0.11466729621260666 0.0 11 0.0 0.0 0.0 0.12815756635526626 0.0 12 0.0 0.0 0.0 0.12815756635526626 0.0 13 0.0 0.0 0.0 0.12815756635526626 0.0 14 0.0 0.0 0.0 0.13321641765876363 0.0 15 0.0 0.0 0.0 0.13658898519442852 0.0 16 0.0 0.0 0.0 0.15682439040841792 0.0 17 0.0 0.0 0.0 0.15682439040841792 0.0 18 0.0 0.0 0.0 0.16019695794408284 0.0 19 0.0 0.0 0.0 0.17705979562240734 0.0 20 0.0 0.0 0.0 0.2090991872112239 0.0 21 0.0 0.0 0.0 0.23607972749654313 0.0 22 0.0 0.0 0.0 0.27823682169235436 0.0 23 0.0 0.0 0.0 0.31027621328117094 0.0 24 0.0 0.0 0.0 0.4047081042797882 0.0 25 0.0 0.0 0.0 0.4097669555832856 0.0 26 0.0 0.0 0.0 0.411453239351118 0.0 27 0.0 0.0 0.0 0.4586691848504266 0.0 28 0.0 0.0 0.0 0.5176891167245624 0.0 29 0.0 0.0 0.0 0.564905062223871 0.0 30 0.0 0.0 0.0 0.6188661427945095 0.0 31 0.0 0.0 0.0 0.6761997909008128 0.0 32 0.0 0.0 0.0 0.7807493845064247 0.0 33 0.0 0.0 0.0 0.8549458702910526 0.0 34 0.0 0.0 0.0 0.994907423021146 0.0 35 0.0 0.0 0.0 1.1196924218407474 0.0 36 0.0 0.0 0.0 1.3675761357121177 0.0 37 0.0 0.0 0.0 1.7520488347779164 0.0 38 0.0 0.0 0.0 2.1837374793430238 0.0 39 0.0 0.0 0.0 2.618798691443796 0.0 40 0.0 0.0 0.0 3.203939158881657 0.0 41 0.0 0.0 0.0 3.635627803446764 0.0 42 0.0 0.0 0.0 4.165120906546154 0.0 43 0.0 0.0 0.0 4.703045428484705 0.0 44 0.0 0.0 0.0 5.240969950423257 0.0 45 0.0 0.0 0.0 5.984621092037368 0.0 46 0.0 0.0 0.0 6.44666284442346 0.0 47 0.0 0.0 0.0 6.957606826076693 0.0 48 0.0 0.0 0.0 7.310040133553675 0.0 49 0.0 0.0 0.0 7.723179656672625 0.0 50 0.0 0.0 0.0 8.100907220667095 0.0 51 0.0 0.0 0.0 8.488752487268558 0.0 52 0.0 0.0 0.0 8.918754848065833 0.0 53 0.0 0.0 0.0 9.39765943813025 0.0 54 0.0 0.0 0.0 9.73660247546457 0.0 55 0.0 0.0 0.0 10.06711409395973 0.0 56 0.0 0.0 0.0 10.399311996222725 0.0 57 0.0 0.0 0.0 10.691039088057739 0.0 58 0.0 0.0 0.0 10.984452463660585 0.0 59 0.0 0.0 0.0 11.304846379548751 0.0 60 0.0 0.0 0.0 11.512259282992142 0.0 61 0.0 0.0 0.0 11.73147617281036 0.0 62 0.0 0.0 0.0 11.994536440592222 0.0 63 0.0 0.0 0.0 12.262655559677583 0.0 64 0.0 0.0 0.0 12.478499881960136 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCTGA 15 0.0022144855 70.0 6 TGTGCGC 15 0.0022144855 70.0 3 GACTAAT 15 0.0022144855 70.0 26 GCGCGTG 20 0.006919731 52.5 6 GGTTTTG 20 0.006919731 52.5 17 CCCGCCG 20 0.006919731 52.5 14 TCTGCGG 20 0.006919731 52.5 3 TCTGCCT 20 0.006919731 52.5 9 AAAATAC 20 0.006919731 52.5 25 GTGCGCG 20 0.006919731 52.5 4 GTAATAT 20 0.006919731 52.5 23 AAAAAGG 130 0.0 51.153847 70 GTATCAT 200 0.0 50.749996 57 CGTATCA 200 0.0 50.749996 56 TATCATT 205 0.0 49.512196 58 ATCATTA 220 0.0 49.31818 59 CCGTATC 210 0.0 48.333336 55 GCCGTAT 210 0.0 48.333336 54 TCGGTGG 225 0.0 48.22222 45 CGGTGGT 240 0.0 48.125 46 >>END_MODULE